Plant regeneration of <i>Rhabdadenia Ragonesei</i> (Apocynaceae) by <i>in vitro</i> culturing of leaf explants

Authors

  • Eduardo Flachsland Instituto de Botánica del Nordeste (IBONE). Sargento Cabral 2131. Corrientes (3400). Argentina
  • Aurelio Schinini Instituto de Botánica del Nordeste (IBONE). Sargento Cabral 2131. Corrientes (3400). Argentina Miembro Carrera de Personal de Apoyo del CONICET
  • Graciela Terada Instituto de Botánica del Nordeste (IBONE). Sargento Cabral 2131. Corrientes (3400). Argentina Facultad de Ciencias Agrarias (UNNE)
  • Graciela Furst Facultad de Ciencias Agrarias (UNNE) Becaria de Prestación de Servicios
  • Luis A. Mroginkski Instituto de Botánica del Nordeste (IBONE). Sargento Cabral 2131. Corrientes (3400). Argentina

DOI:

https://doi.org/10.30972/bon.141-21391

Abstract

Plants of Rhabdadenia Ragonesei Woodson (Apocynaceae) were regenerated in vitro from leaves explants. The procedure employed includes: 1) Surface sterilization of leaves by immersion in 70% ethanol (10 s) followed by 1,1%NaOCl (15 min) and three wash with sterile distilled water. 2) Callus and buds induction by culture on Murashige and Skoog medium (MS) + 3 mg/L benzyladenine (BAP). 3) Subculture of callus and buds on MS + 1 mg/L BAP, and 4) Rooting on MS + 0.5 mg/L naftalenacetic acid

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Published

2005-01-01

How to Cite

Flachsland, E., Schinini, A., Terada, G., Furst, G., & Mroginkski, L. A. (2005). Plant regeneration of <i>Rhabdadenia Ragonesei</i> (Apocynaceae) by <i>in vitro</i> culturing of leaf explants. Bonplandia, 14(1-2), 91–96. https://doi.org/10.30972/bon.141-21391

Issue

Vol. 14 No. 1-2 (2005)

Section

Original papers

License

Declaration of Adhesion to Open Access

  • All contents of Bonplandia journal are available online, open to all and for free, before they are printed.

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