Cinética de consumo de sustratos orgánicos de bacterias aisladas de la Laguna de Chascomus

Autores/as

  • María C. Romero
  • Aldo A. Mariazzi Instituto de Limnología "Dr. Raúl Á. Ringuelet"

DOI:

https://doi.org/10.30972/eco.16277071

Resumen

Eight bacterial strains were isolated from Chascomús Pond (Buenos Aires Province) and their capacity to growth at low concentrations of glycolate and glucose, as a solé source of organic carbón was evaluated.
Substrate affinities (Kt valúes) and other kinetic parameters were measured by Wright & Hobbie methodology with 14C-labelled glycollate and MC-labelled glucose as substrates. Four o f the eight puré culture studied followed Michaelis-Menten uptake
kinetics giving good straight lines after the Lineweaver-Burke transfonnation.
Vmax valúes o f natural popuiations and the experimental ones were similar for glycollate; for glucose the experimental valúes were a little higher. This suggest the possibility o f applying the experimental Kt valúes to natural popuiations as a way of evaluation o f the natural substrate concentration.

Citas

AKAGI, Y., TAGA, N. & SIMIDU, V. 1977. Isolation and distribution of ligotrophic bacteria. Can J. Microbio!. 23:981-987.

AKAGI, Y. & TAGA, N. 1980. Uptake o f D-glucose and L-proline by oligotrophic and heterotrophic maeine bacteria. Can. J. Microbiol. 26:454-459.

CONZONNO, V. H. & FERNANDEZ CIRELLI, A. 1985. Soluble humic substances from the affluents o f Chascomús Pond (Argentina), farch. Hydrobiol. 109: 305-314.

CONZONNO, V. H. & CLAVERIE, E.- F. 1990. Chemical characteristics of the water of Chascomús Pond (Provincia de Buenos Aires, Argentina). Limnological implications. Rev. Brasil. Biol. 50: 15-21.

DANGAUS, N. V. 1976. Descripción sistemática de los parámetros morfológicos considerados en las lagunas pampásicas. Limnobios. 1: 35-39.

DOWD, J. E. & RIGGS, D. S. 1965. A comparison o f estimates o f Michaelis Mentes kinetics constants form various linear transformations. J. Bio. Chem. 240: 863-869.

HAMILTON, R. D. & PRESLAN, J. E. 1970. Observations o f heterotrophic activity in the eastem tropical Pacific. Limnol. Oceanogr. 15: 394-401.

ISHIDA, Y . & KADOTA, H. 1981. Grownth pattems and substrate requirements of naturally occurring oblígate oligotrophs. Microb. Ecol. 7: 123-130.

ISHIDA, Y., IMAI, I. MIYAGAKI, T. & KADOTA, H. 1982. Growth and uptake kinetics of a facultatively oligotrophic bacterium at low nutrient concentrations. Microb. Ecol. 8: 23-32.

KOOIJ, D., ORANJE, J. P. & HIJNEN, A. M. 1982. Growth o f Pseudomonas aeruginosa in tap water in relation to utilizat ion of substrates at concentrations of a few micrograms per liter. Appl. Environ. Microbio. 44:1086-1095.

HOSHLAND, D. E.,NEM ETLY,G. &FILMER.D. 1966. Comparison of experimental binding data and the theoretical models in proteins containing subunits. Biochem. 5:365-385.

MARTIN, P. & MAC LEOD, R. A. 1984. Observations on the distinction between oligotrophic and eutrophic marine bacteria. Appl. environ. Microbiol. 47:1017-1022.

MOALEDJ, K. 1978. Qualitative analysis o f an oligocarbophiclic microflora in the Plubsee. Arch. Hydrobiol. 82:98-113.

MOALEDJ, K. & OVERBECK, J. 1980. Studies on uptake kinetics of olicarbophilic bacteria. Arch Hydrobio. 89: 303-312.

OVERBECK, J. 1979. Studies on heterotrophic functions and glucose metabolism of microplankton in Pluosee. Arch. Hydrobiol. Beich. Ergebn. Limnol. 13:56-57.

OVERBECK, J. & WITZEL, K. P. 1975. New approaches for measuring microbial activities in the lake ecosystem. Proc. lst. Int. Congr. IAMS 2:222-228.

ROMERO, M.C. & MARIAZZI, A. A. 1985. Heterotrophic bacterial uptake on glucose and glycollate in an eutrophic pond (Chascomús, Argentina). Ecosurr 12/13:111-121.

TANAKA, A., NAKANISHI, Mi. & KADOTA, H. 1975. Distribution and activity of glycollate utilizing bacteria in the water column in Lake Biwa. Bull. Jap. Soc. Scient. Fish. 41:251-256.

VACCARO, R. F. & JANNASCH, H. W. 1966. Studies on heterotrophic actiyity in seawater based on glucose assiinilation. Limnol. Oceanogr. 11:596-607.

VACCARO, R. F. & JANNASCH, H. W. 1967. Variations in uptake kinetics for glucose by natural populations in seawater. Limnol. Oceanogr. 18:159-165.

WILLIAMS, P. J. 1973. The validity o f the application o f simple kinetic analysis to heterogenous microbial populations. Limnol. Oceanogr. 18:159-165.

WRIGHT, R. T. & HOBBIE, J. E. 1965. The uptake of oiganic solutes on lake water. Limnol. Oceanogr. 10: 22-28.

WRIGHT, R. T. & HOBBIE, J. E. 1966. Use of glucose and acétate by bacteria and algae in aquatic ecosystems. Ecology 47: 447-464.

WRIGHT, R. T. 1970. Glycollate acid uptake by planktonic bacteria. En D. W. Hood (ed.) organic matter in natural waters pp. 521-536. Inst. Mar. Sci. Alaska.

WRIGHT, R. T. 1973. Some difficulties in using MC-organic solutes to measure heterotrophic bacterial activity. En H.L. Stevenson & R. R. Colwell (eds.) Belle W. Baruch Library in Marine Science. Vol. 1 Estuarine Microbial Ecology. Univ. South Carolina Press., Columbia, South Carolina.

Descargas

Publicado

2023-11-29 — Actualizado el 2023-11-29

Versiones

Cómo citar

Romero, M. C., & Mariazzi, A. A. (2023). Cinética de consumo de sustratos orgánicos de bacterias aisladas de la Laguna de Chascomus. Ecosur, 16(27), 31–43. https://doi.org/10.30972/eco.16277071

Número

Sección

Artículos