Cinética de consumo de sustratos orgánicos de bacterias aisladas de la Laguna de Chascomus
DOI:
https://doi.org/10.30972/eco.16277071Resumen
Eight bacterial strains were isolated from Chascomús Pond (Buenos Aires Province) and their capacity to growth at low concentrations of glycolate and glucose, as a solé source of organic carbón was evaluated.
Substrate affinities (Kt valúes) and other kinetic parameters were measured by Wright & Hobbie methodology with 14C-labelled glycollate and MC-labelled glucose as substrates. Four o f the eight puré culture studied followed Michaelis-Menten uptake
kinetics giving good straight lines after the Lineweaver-Burke transfonnation.
Vmax valúes o f natural popuiations and the experimental ones were similar for glycollate; for glucose the experimental valúes were a little higher. This suggest the possibility o f applying the experimental Kt valúes to natural popuiations as a way of evaluation o f the natural substrate concentration.
Citas
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