Búsqueda de actividad biológica de aislados de fitoproteasas de Bromeliaceae nativas y cultivadas en Corrientes sobre microorganismos fitopatógenos
DOI:
https://doi.org/10.30972/bon.3417883Palavras-chave:
Ananas comosus, Bromelia serra, control naturalResumo
El objetivo de este estudio es evaluar la actividad biológica de aislados proteicos de la planta nativa Bromelia serra (BS) y cultivada Ananas comosus (AC) sobre microorganismos fitopatógenos. Se obtuvieron muestras de bromeliáceas cultivadas (AC) del Campo Experimental de la Facultad de Ciencias Agrarias (UNNE) y de la bromeliácea nativa BS de montes naturales de la región. Se prepararon extractos de tallo y hojas de AC y hojas de BS, los cuales después de ser precipitados con acetona y obtener pellets, fueron resuspendidos en buffer estéril, los cuales se denominaron aislados proteicos. Se evaluó la actividad antimicrobiana frente a bacterias fitopatógenas mediante la técnica de difusión en disco. Sin embargo, no se observó la formación de un halo de inhibición en ninguno de los tratamientos con aislados proteicos, a diferencia del control positivo con estreptomicina. Se realizó la concentración inhibitoria mínima de crecimiento bacteriano por la técnica de microdilución en caldo. Los resultados mostraron que los aislados proteicos no inhibieron el crecimiento bacteriano. Además, se evaluó la actividad antifúngica contra Fusarium oxysporum mediante la técnica del MTT, pero tampoco se observó inhibición. Aunque los extractos de proteínas no mostraron actividad biológica contra los microorganismos evaluados, futuros experimentos continuarán la evaluación utilizando otros microorganismos. Además, una vez que se establezca un esquema de purificación, se utilizarán diferentes condiciones operativas y pruebas con enzimas puras en lugar de sus extractos.
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