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Diet and histomorphological study of the gastrointestinal system of melanophryniscus klappenbachi (anura: bufonidae)

A. M. Arias, D. D. Larrea, J. A. Céspedez, C. Quintana, G. B. Olea
Idioma: en


The diet and histomorphology of the digestive tract of Melanophryniscus klappenbachi were determined by the analysis of adult and juvenile specimens collected in a private field in Bermejo department, Chaco, Argentina. The sampling was carried out monthly from March to June 2015. 29 specimens were collected, measured, sexed, and dissected for the obtainment of the digestive content and his- tological samples. The results showed a high tendency towards myrmecophagy; more than 95% of the recovered prey items were identified as ants. The histomorphology of the gastrointestinal system consisted of the main four layers of tissue observed in vertebrates: mucosa, submucosa, muscular, and serosa. This study constitutes a contribution to the knowledge of biology and the natural history of anurans of the Bufonidae family, especially the genus Melanophryniscus, which currently receives a great amount of attention regarding its con- servation status.

Palabras clave

Histology of digestive tract; klappenbach’s frogs; myrmecophagy


Akat E, Arikan H, Gorm en, B. 2014. Histochemical and biometric study of the gastrointestinal system of Hyla orientalis (Bedriaga, 1890) (Anura, Hylidae). Europ JH istochem 58: 291-295.

Akat E. 2019. Histological and histochemical aspects of the digestive tract of Lyciasalamandra billae arikani G09- men & Akman, 2012 (Urodela: Salamandridae). Acta Zool Bulg 71: 525-529.

Anderson AM, Haukos DA, Anderson J. 1999. Diet composition of three anurans from the playa Wetlands of Northwest Texas. Copeia 2: 515-520.

Bonansea M I, Vaira M. 2007. Geographic variation of the diet of Melanophryniscus rubriventris (Anura: Bufonidae) in Northwestern Argentina. J Herpet 41: 231-236.

Bortolini SV, Maneyro R, Coppes F, Zanella N. 2013. Diet of Melanophryniscus devincenzii (Anura: Bufonidae) from Parque Municipal de Sertao, Rio Grande do Sul, Brazil. The Herpet J 23: 115-119.

Brasileiro CA, Marins M, Sazim a I. 2010. Feeding ecology of Thoropa taophora (Anura: Cycloramphidae) on a rocky seashore in southeastern Brazil. South Am J Herpetol 5: 181-188.

Cakici O, A kat E. 2013. Some histomorphological and histochemical characteristics of the digestive tract of the snake-eyed lizard, Ophisops elegans Menetries, 1832 (Squamata: Lacertidae). North-Western J Z o o l 9: 257-263.

Cramp R L, Franklin CE. 2005. Arousal and re-feeding rapidly restores digestive tract morphology following aestivation in green-striped burrowing frogs. Comp Biochem & Physiol-Part A: Molec & IntegratPhysiol 142: 451-460.

Daly JW et al. 2008. Indolizidine 239Q and quinolizidine 275I. Major alkaloids in two Argentinian bufonid toads (Melanophryniscus). Toxicon 52: 858-870.

Di Rienzo JA et al. 2015. InfoStat versión 2015. Grupo InfoStat, FCA, Universidad Nacional de Córdoba, Argentina.

Domeneghini C, Arrighi S, Radaelli G, Bosia G, Veggetti A. 2005. Histochemical analysis of glycoconjugate secretion in the alimentary canal of Anguilla anguilla L. Acta Histochem 106: 477-487.

Donnelly MA. 1991. Feeding patterns of the strawberry poison frog, Dendrobates pumilio (Anura: Dendrobatidae). Copeia 1991: 723-730.

Duré M, Kehr AI. 2006. Melanophryniscus cupreuscapularis (NCN). Diet, short notes. Herpetol Rev 37: 338.

Duré MI, Kehr A I, Schaefer EF. 2009. Niche overlap and resource partitioning among five sympatric bufonids (Anura, Bufonidae) from northeastern Argentina. Phyllomedusa 8: 27-39.

Feder ME. 1992. Aperspective on the environmental physiology of the amphibians. In Feder M.E., Burggren W.W. (Eds.), Environmental Physiology of the Amphibians, The University of Chicago Press, Chicago, p.1-6.

Ferri D, Liquori GE, Natale L, Santarelli G, Scillitani G. 2001. Mucin histochemistry of the digestive tract of the red-legged frog Rana aurora. Acta Histochem 103: 225¬ 237.

Fry AE, Kaltenbach JC . 1999. Histology and lectin binding patterns in the digestive tract of the carnivorous larvae of the Anuran, Ceratophrys ornata. JM orphol 241: 19-32.

Guiurca D, Zaharia L. 2005. Data regarding the trophic spectrum of some population of Bombina variegata from Bacau county, North-Western. J Zool 1: 15-24.

Hammer O, Harper DA, Ryan PD. 2001. Paleontological statistics software package for education and data analysis. Palaeontol Electron 4: 1-9.

Ishizuya OA, Ueda S. 1996. Apoptosis and cell proliferation in the Xenopus small intestine during metamorphosis. Cell & Tissue Research 286: 467-476.

Kaltenbach JC , Fry AE, Colpitts KM, Faszewski EE. 2012. Apoptosis in the digestive tract of herbivorous Rana pipiens larvae and carnivorous Ceratophrys ornata larvae: an immunohistochemical study. J Morphology 273: 103-108.

Kwet A, Maneyro R, Zillikens A, Mebs D. 2005. Advertisement calls of Melanophryniscus dorsalis (Mertens, 1933) and M . montevidensis (Philippi, 1902), two parapatric species from southern Brazil and Uruguay, with comments on morphological variation in the Melanophryniscus stelzneri group (Anura: Bufonidae). Salamandra 41: 1-18.

Lajm anovich RC. 1995. Relaciones tróficas de bufónidos (Anura, Buronidae) en ambientes del Río Paraná, Argentina. Alytes 13: 87-103.

Liquori GE Scillitani G, Mastrodonato M, Ferri D. 2002. Histochemical investigations on the secretory cellsin the oesophagogastric tract of the Eurasian green toad, Bufo viridis. Histochem J 34: 517-524.

Liquori GE, Mastrodonato M, Zizza S, Ferri D. 2007. Glycoconjugate histochemistry of the digestive tract of Triturus carnifex (Amphibia, Caudata). J Molec Histol 38:191-199.

López JA, Scarabotti PA, Medrano MC, Ghirardi R. 2009. Is the red spoted green frog Hypsiboas punctatus (Anura: Hylidae) selecting its preys? The importance of prey availability. Rev de Biol Trop 3: 847-857.

Machado SC, Pelli AA, Abidu FM, Debrito GL. 2014. Histochemical and immunohistochemical analysis of the stomach of Rhinella icterica (Anura, Bufonidae). JH istol 2014: 1-8.

Maneyro R, Naya DE, Rosa I, Canavero A, Camargo A. 2004. Diet of the South American frog Leptodactylus ocellatus (Anura, Leptodactylidae) in Uruguay. Iheringia, Série Zoologia 94: 57-61.

Maneyro R, Kwet A. 2008. Amphibians in the border region between Uruguay and Brazil: updated species list with comments on taxonomy and natural history (Part I: Bufonidae). Stutgarter Beitrage zur Naturkunde 1: 95-121.

Mebs D, Pogoda W, Maneyro R, Kwet A. 2005. Studies on the poisonous skin secretion of individual red bellied toads, Melanophryniscus montevidensis (Anura, Bufonidae), from Uruguay. Toxicon 46: 641-650.

Muikham I, Srakaew N, Chatchavalvanich K, Chumnanpuen P. 2016. Microanatomy of the digestive system of Supachai’s caecilian, Ichthyophis supachaii Taylor, 1960 (Amphibia: Gymnophiona). Acta Zoologica 98: 252-270.

Scott NJ, Woodward BD. 2001. Relevamientos de lugares de reproducción. En: Medición y monitoreo de la diversidad biológica, métodos estandarizados para anfibios, Heyer W.R. el al. Ed. Univ.Patagonia, Chubut , p. 113-117.

Shannon CE, Weaver W. 1949. The mathematical theory of communication. Univ. Illinois Press, Urbana, Illinois Press, 125 p.

Suganum a T et al. 1981. Comparative histochemical study of alimentary tracts with reference to the mucous neck cells of the stomach. Am J Anatomy 161: 219-238.

Taigen T, Wells K. 1985. Energetics of vocalization by an anuran amphibian (Hyla versicolor). Journal of Comparaive Physiology. Biochemical, systemic, and environmental physiology 2: 163-170.

DOI: http://dx.doi.org/10.30972/vet.3225718