Estudio del glicoconjugado glucosa/manosa en mucosa gástrica de cerdos diagnosticados con Helicobacter pylori

M P. Vandeerveen; S. R. Giménez; M. C. Grosso; F. U. Bonino; V. H. Macloughlin

doi:10.30972/vet.3215631

Authors

M P. Vandeerveen Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra Histología.
S. R. Giménez Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra Histología.
M. C. Grosso Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra Histología.
F. U. Bonino Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra Bioestadística.
V. H. Macloughlin Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra Histología.

DOI:

https://doi.org/10.30972/vet.3215631

Keywords:

Helicobacter pylori, pigs, gastric mucosa, lectin-histochemistry, glucose/mannose

Abstract

Helicobacter pylori, a Gram negative bacillus, is associated with gastritis, ulcer, and gastric cancer. The worldwide prevalence of H. pylori infection is greater than 50%, constituting a public health problem. Different species of Helicobacter were described, which affect both animals and humans, and may be responsible for zoonoses. The colonization process of this bacterium depends largely on its binding to glycoconjugates present in the gastric mucosa of the host. The pig represents the animal model of choice for the study of infection by this bacterium. The objective of the present work was to analyze the behavior of the glucose/mannose glycoconjugate in the gastric mucosa of pigs affected by gastritis caused by Helicobacter sp. Samples from the antral region of the stomach of crossbred pigs were used, obtained from slaughterhouse in the Río Cuarto (Argentina). The samples were classified into different study groups according to the type of gastritis and the presence or absence of Helicobacter sp. A lectin histochemistry study for glucose/mannose glycoconjugate was carried out, the evaluated under microscope and statistical analysis performed. The results obtained indicate that there were no significant differences between the groups in the glucose/mannose expression in epithelium and gastric glands, but there were significant differences in the expression of this glycoconjugate in lamina propria between Helicobacter sp positive acute gastritis with respect to the Helicobacter sp negative acute gastritis groups and with normal mucosa group. This results reveal that there is relationship between the increase in glucose/mannose expression and the presence of Helicobacter sp in the lamina propria of pig gastric mucosa.

Downloads

Download data is not yet available.

References

Akopyants NS, Eaton KA, Berg DE. 1995. Adaptative mutation and colonization during Helicobacter pylori infection of gnotobiotic piglets. Infect Imm 63: 116-121.

Artis D, Grencis RK. 2008. The intestinal epithelium: sensors to effectors in nematode infection. Mucosal Immun 1: 4, 252–264.

Baczako K, Kuhl P, Malfertheiner P. 1995. Lectin-binding properties of the antral and body surface mucosa in the human stomach are the difference revelant for Helicobacter pylori affinity?. Journal Pathol 176: 77-86.

Baele M et al. 2008. Isolation and characterization of Helicobacter

suis sp. nov. from pig stomachs. Int J Syst Evol Microbiol 58: 1350-1358.

Baele M et al. 2009. Non-Helicobacter pylori helicobacters detected in the stomach of humans comprise several naturally occurring Helicobacter species in animals. FEMS Immun Med Microbiol 55: 306-313.

Debenedetti MA et al. 2018. Determinación citoinmunohistoquímica

de células D en estómago de cerdos con Helicobacter spp. Ab Intus 1: 2, 39-46.

Delacruz J et al 2001. Application of lectins for the identification

of glycoconjugate in the large intestine of chinchilla (Chinchilla lanigera). Biocell: 25: 1, 139.

Gimeno EJ, Barbeito CG. 2004. Glicobiología, una nueva dimensión para el estudio de la biología y de la patología. Anales Acad Nac Agron & Vet LVIII 58: 6-34.

Guendulain C, Sibilla ML. 2018. Helicobacter gástricos en perros y gatos y su significancia en la salud humana. Ab Intus 2: 1, 93-100.

Haesebrouck F et al. 2009. Gastric Helicobacters in domestic

animals and nonhuman primates and their significance for human health. Clin Microbiol Rev 22: 2, 202-223.

Hernández PE. 2010. Bacterias patógenas emergentes transmisibles por los alimentos. http://www.analesranf.com/index.php/mono/article/viewFile /1111/1128

Joosten M et al. 2013. Case report: Helicobacter suis infection

in a pig veterinarian. Helicobacter 18: 392-396.

Krakowka S, Eaton KA, Rings DM. 1995. Occurrence of gastric ulcer in gnotobiotic piglets colonized by Helicobacter pylori. Infec Immun 63: 2352-2355.

Kusters JG, Vliet AM, Kuipers EJ. 2006. Pathogenesis of Helicobacter pylori infection. Clin Microbiol Rev 19: 3, 449-490.

León BR, Recavarren S, Ramirez RA. 1991. El aporte peruano a la investigación sobre Helicobacter pylori. Rev Méd Herediana 4: 173-181.

Liang J et al. 2013. Sequence typing of porcine and human gastric pathogen Helicobacter suis. Int J Syst and Evol Microbiol 51: 3, 920-936.

Lueth M et al. 2005. Lectinhistochemistry of the gastric mucosa in normal and Helicobacter pylori infected guinea-pigs. J Mol Hist 36: 51-58.

Melo MR, Cavalcanti C, Pontes FN, Carvalho L, Beltrao E. 2008. Patrones de tinción de lectina en la mucosa gástrica humana con y sin exposición a Helicobacter pylori. J Braz Microbiol 39: 2, 238-240.

Morales BA, Bermúdez V. 2008. Modelos animales para el estudio de la infección por el género Helicobacter en humanos. Rev Soc Med Quir Hosp Emerg Perez de León 39: 1, 30-33.

Morales MR, Castillo RG, López VY, Cravioto A. 2019. Helicobacter pylori. http://www.biblioweb.tic.unam.mx/libros/microbios/Cap11/. ISBN 968-36-8879-9.

Paredes LE. 2013. Glicoconjugados en epitelios. Sistema de Revisión en Investigación Veterinaria de San Marcos UPG, 1-9.

Polanco R et al. 2006. Lesiones gástricas asociadas a la presencia de bacterias del Género Helicobacter en caninos. Rev Científ FCV-LUZ 6: 16, 585-592.

Quintana MP, Padra M, Padra JT, Benktander J, Linden SK. 2018. Mucus pathogen interactions in the gastrointestinal tract of farmed animals. Micro-organisms 6: E55.

Rivas TF, Hernandez F. 2000. Helicobacter pylori: factores de virulencia, patología y diagnóstico. Rev Biomed: 11: 187-207.

Rodríguez B, Aranzazu D, Ortiz L. 2008. Determinación de Helicobacter spp en cerdos en el depto.de Antioquia, Colombia. Rev Colom Cien Pec 21: 2, 210-218.

Rodríguez B, Aranzazu D, Ortiz L. 2009. Association of gastric ulcer and Helicobacter sp in pigs in Antioquia, Colombia. Rev Colom Cien Pec 22: 54-60.

Selam B, Kayisli UA, Mulayim N, Arici A. 2001. Regulation of fas ligand expression by estradiol and progesterone in human endometrium. Biol Reprod 65: 4, 979-985.

Torres F, Torres C. 2016. Fisiopatología molecular en la infección por Helico- bacter pylori. Salud Uninorte 32: 3, 500-512.

Vandeerveen MP et al. 2018. Determinación de L-fucosa en epitelio gástrico de cerdos con Helicobacter sp. Estudio preliminar. Morfovitual 2018. http://www.morfo-virtual2018.sld.cu/index.php/morfovirtual/2018/paper/view/212

Vandeerbulck K et al. 2005. Identification of non-Helicobacter

pylori spiral organisms in gastric samples from humans, dogs, and cats. Journal Clin Microbiol 43: 2256-2260.