Resistencia antimicrobiana de Staphylococcus aureus y Streptococcus agalactiae aislados de leche de vacas con mastitis

R. Quispe; G. Peña; V. Andía

doi:10.30972/vet.3215640

Authors

R. Quispe Universidad Nacional de San Cristóbal de Huamanga. Facultad de Ciencias Biológicas. Laboratorio de Microbiología de Alimentos y Laboratorio de Biología Celular y Molecular.
G. Peña Universidad Nacional de San Cristóbal de Huamanga. Facultad de Ciencias Biológicas. Laboratorio de Microbiología de Alimentos y Laboratorio de Biología Celular y Molecular.
V. Andía Universidad Nacional de San Cristóbal de Huamanga. Facultad de Ciencias Biológicas. Laboratorio de Microbiología de Alimentos y Laboratorio de Biología Celular y Molecular.

DOI:

https://doi.org/10.30972/vet.3215640

Keywords:

bovine mastitis, milk, Staphylococcus aureus, Streptococcus agalactiae, antimicrobial resistance

Abstract

The indiscriminate use of antibiotics without prior in vitro sensitivity test is the main cause of failure for the treatment of mastitis in the Ayacucho region. In order to assess the antimicrobial resistance of Staphylococcus aureus and Streptococcus agalactiae causing bovine mastitis, the isolation, identification and antibiogram of the bacteria under study were carried out to find the resistance against five antibiotics. Samples were collected at the Allpachaca farm that belongs to the National University of San Cristobal de Huamanga (Ayacucho, Perú), located at coordinates 13°23’30.79 “S; 74° 15’56.48 “W, at an altitude of 3528 meters above sea level. In the field, 94 dairy cows were studied using the California Mastitis Test (CMT), which resulted in 35 cows with subclinical mastitis. In the laboratory, the isolation and the identification of S. aureus and S. agalactiae were carried out, later the antibiogram was carried out using the method of Bauer et al. (1966). Then 35.2% of S. aureus and 12.7% S. agalactiae were isolated. The results of the antibiogram show that S. agalactiae was 22% resistant to penicillin, 22% to cephalexin and 33.3% sulfatrimethoprim, whereas tetracycline, amoxicillin plus clavulanic acid were 100% sensitive. On the other hand, .S. aureus was 52% resistant to penicillin and amoxicillin plus clavulanic acid 28%, while cephalexin, tetracycline and sulfatrimethoprim were 100% sensitive.

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