Efecto de dos medios de capacitación espermática (B.O vs Percoll) en la fecundación in vitro de ovocitos bovinos

L. Tinoco; E. D. Capa Mora; R. Carrera

doi:10.30972/vet.3517479

Authors

L. Tinoco Universidad Técnica Particular de Loja https://orcid.org/0000-0003-4651-2274
E. D. Capa Mora Universidad Técnica Particular de Loja https://orcid.org/0000-0003-2880-1873
R. Carrera Universidad Técnica Particular de Loja https://orcid.org/0000-0002-4606-5219

DOI:

https://doi.org/10.30972/vet.3517479

Keywords:

capacitation, motility, embryonic development, in vitro fertilization, percoll

Abstract

The study aimed to evaluate the Fertilization Rate of four independent in vitro treatments of bovine oocytes with Sexed (SX) and Conventional (Conv) semen, capacitated (B.O and Percoll) using the Swim-Up technique. Cleavage was assessed at 48 hours post-fertilization and on the seventh day of embryonic development. The results showed a cleavage percentage of 59.4% in T1 (Conv. Percoll), 60% in T2 (Conv. B.O), 75% in T3 (SX. Percoll), and 46.6% in T4 (SX. B.O), with a statistically significant difference (p>0.05) observed specifically between T3 and T4. The embryo development rate on the seventh day was 34.4% in T1, 20% in T2, 15% in T3, and 10.51% in T4. In this case, a statistically significant difference was found in the use of conventional medium with Percoll compared to SX B.O medium (p<0.05) (T1 and T4, respectively). The results also showed similar production of morula and early blastocysts and protruded embryos among the four treatments, demonstrating the feasibility of in vitro fertilization (IVF) technique for producing bovine embryos. In conclusion, the preliminary evaluation highlights the significant influence of the sexing method and treatment on semen quality and embryonic development in animal reproduction programs. Although Sexed semen with Percoll shows potential to improve cleavage rates, conventional semen, especially in T1 treatment (Conv. Percoll), exhibits superiority in individual motility and concentration. These findings provide crucial insights for optimizing reproductive programs.

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