Diagnóstico molecular del virus de laringotraqueítis infecciosa aviar en granjas de gallinas ponedoras localizadas en Tungurahua, Ecuador

M. Revelo-Cueva; C.V. Vinueza-Burgos; G.E. Metz; M.G. Echeverría

doi:10.30972/vet.3527866

Authors

M. Revelo-Cueva Universidad Central del Ecuador. Facultad de Medicina Veterinaria y Zootecnia https://orcid.org/0000-0002-5713-0020
C.V. Vinueza-Burgos Universidad Central del Ecuador. Facultad de Medicina Veterinaria y Zootecnia https://orcid.org/0000-0002-4893-502X
G.E. Metz Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias https://orcid.org/0000-0001-5098-7059
M.G. Echeverría Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias https://orcid.org/0000-0001-9644-3583

DOI:

https://doi.org/10.30972/vet.3527866

Keywords:

Commercial hens, Sequences, Vaccines, Thymidine kinase, ICP4

Abstract

Avian infectious laryngotracheitis (ILT) is a disease caused by Gallid alphaherpesvirus 1 (GaHV-1), which is transmitted through respiratory secretions or fomites from infected birds, either naturally or through the use of chicken embryo-origin (CEO) live vaccines. The objective of this research was to develop a molecular detection of GaHV-1 in laying hen farms located in Tungurahua Province. Laryngotracheal swabs and tracheal samples were collected from 47 farms. In addition, a recombinant vaccine with GaHV-1 glycoprotein B was used. Three farms tested positive to GaHV-1 as they amplified segments of the ICP4 and thymidine kinase (TK) genes. As expected, the recombinant vaccine analyzed was negative to the amplification of the studies genes. The amplification of the ICP4 and TK genes in the field samples demonstrated the presence of GaHV-1 in the province, but the possibility that it was a vaccine-derived virus from the use of the recombinant vaccine authorized for use in the study area was ruled out. Since the birds did not present clinical signs related to ILT, it was determined that an outbreak of the disease could not be confirmed. However, a molecular study with full genome sequencing is required to obtain conclusive results. However, the use of CEO-type vaccines should be avoided in vaccination schedules in Tungurahua Province, and only the use of vectorized vaccines authorized by the national regulatory authority should continue.

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