Eficacia de SNAP DUO ST Plus y HPLC en la detección de residuos de antibióticos en leche cruda del Valle del Mantaro, Perú

F. A. Villar; L. G. Estremadoyro; I. U. Payano; N. M. Sanchez; K. A. Laurente; Y. Maurico-Ramos; J. N. Carhuas

doi:10.30972/vet.3617987

Authors

F. A. Villar Universidad Nacional del Centro del Perú. Departamento Académico de Zootecnia https://orcid.org/0000-0002-1768-8388
L. G. Estremadoyro Universidad Nacional del Centro del Perú. Departamento Académico de Zootecnia https://orcid.org/0000-0002-3756-7516
I. U. Payano Universidad Nacional del Centro del Perú. Departamento Académico de Zootecnia https://orcid.org/0000-0002-6441-5016
N. M. Sanchez Universidad Nacional del Centro del Perú. Departamento Académico de Zootecnia https://orcid.org/0000-0001-6157-468X
K. A. Laurente Universidad Nacional del Centro del Perú. Departamento Académico de Zootecnia https://orcid.org/0009-0004-5681-7105
Y. Maurico-Ramos Universidad Nacional del Centro del Perú. Departamento Académico de Zootecnia https://orcid.org/0009-0004-5681-7105
J. N. Carhuas Universidad Nacional del Centro del Perú. Departamento Académico de Zootecnia. Universidad Peruana Los Andes. Facultad de Ciencias de la Salud https://orcid.org/0000-0002-0137-0631

DOI:

https://doi.org/10.30972/vet.3617987

Keywords:

Milk diagnostic tests, Rapid screening, Sensitivity, Specificity, Cost- effectiveness

Abstract

The objective of the present study was to determine and compare the efficacy of SNAP DUO ST Plus and HPLC in the detection of antibiotic residues in milk produced to ensure food safety. To this end, raw milk samples from 32 dairy herds and 8 collection centers were evaluated using the SNAP DUO* ST Plus rapid test for tetracyclines and β-lactams, and the high-performance liquid chromatography (HPLC) method. The analysis was conducted in laboratories at the National University of the Center of Peru. A total of 80 milk samples were analyzed, with antibiotic residues found in 37.5% of the samples using SNAP DUO, and in 52.5% using HPLC. The SNAP DUO test showed substantial agreement with HPLC (Kappa=0.734), with a sensitivity and specificity of 89.3% and 95.3%, respectively, compared to 100% for HPLC. In terms of cost-effectiveness, HPLC presented a higher cost per correctly identified result (S/ 20.27) compared to SNAP DUO (S/ 7.24), which was identified as the most efficient method. The implementation of SNAP DUO in antimicrobial residue monitoring programs in milk is recommended due to its effectiveness and efficiency, while HPLC is ideal for research and corroboration of results, contributing to the improvement of food safety in milk consumption.

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