Pérdidas reproductivas asociadas a infecciones bacterianas y protozoarias en rodeos bovinos de la provincia de Corrientes

Paola Della Rosa; Juan Manuel Sala; V. Morel; Daniel Francisco Benítez; Sergio Gastón Caspe

doi:10.30972/vet.3719047

Authors

Paola Della Rosa AER INTA Goya. Av. José Jacinto Rolón 736 (W3450) https://orcid.org/0009-0006-7828-6442
Juan Manuel Sala EEA INTA Mercedes, Juan Pujol al este sin número, (W3470) Mercedes, Corrientes. https://orcid.org/0009-0006-5800-476X
V. Morel CONICET. Godoy Cruz 2290 (C1425FQB) CABA –Argentina
Daniel Francisco Benítez 2EEA INTA Mercedes, Juan Pujol al este sin número, (W3470) Mercedes, Corrientes https://orcid.org/0000-0002-3379-4332
Sergio Gastón Caspe AER INTA Goya. Av. José Jacinto Rolón 736 (W3450), Goya. Moredun Research Institute, Pentland Science Park, EH26 0PZ, Penicuik, UK. https://orcid.org/0000-0001-5631-3557

DOI:

https://doi.org/10.30972/vet.3719047

Keywords:

abortion, reproductive losses, cattle, cervicovaginal mucus

Abstract

The aim of the present study was to determine the association between serological status and the causes of abortion related to bacterial and protozoan infections in three livestock farms with low reproductive performance in Corrientes province, Argentina. During 2020-2021, preputial scraping of bulls, pregnancy diagnosis by transrectal ultrasound in cows, and collection of aborted fetuses were performed. Blood samples from pregnant, non-pregnant and aborted cows were analyzed for Leptospira spp., Neospora caninum and Brucella abortus antibodies. In addition, cervicovaginal mucus (CVM) samples were collected from aborted cows for culture. Five percent of the bulls were positive for Tritrichomonas foetus and 7% for Campylobacter fetus (locality 1). Among 546 sera analysed, 1.8% (10/546; 95% CI 1-3) were positive for Leptospira spp., showing a significant association between high seroprevalence and non-pregnant females (p = 0.0174). For N. caninum, the seroprevalence was 49.1% (268/546 95% CI 45-54), showing a statistically significant association between seropositivity and pregnant females (p = 0.0151). Of 14 aborted females monitored, 42.8% became seropositive to N. caninum, suggesting horizontal transmission, a finding of notable importance. All animals were seronegative for B. abortus. Furthermore, T. foetus (3/61) and C. fetus (3/61) were isolated from 9.8% (6/61) of CVM samples, and infectious agents were identified as the cause of abortion in 42.9% (3/7) of the fetuses. This study provides insight into infectious causes of reproductive losses in extensive cattle herds in the Argentine Mesopotamia, highlighting the importance of venereal disease in bulls and the need for sampling of aborted cows and fetuses to identify causes of reproductive failure.

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References

1. Baumgartner W. Fetal disease and abortion: diagnosis and causes. Bovine reproduction. 2021; 665-716.

2. Camillo G, Cadore G, Cezar AS, Toscan G. Anticorpos anti- Neospora caninum em bovinos de leite do sudoeste do estado do Paraná. Arq. Bras. Med. Vet. Zootec. 2010, 62: 1511-1513.

3. Campero CM, Moore DP, Odeon AC, Cipolla AL, Odriozola E. Aetiology of bovine abortion in Argentina. Vet. Res. Commun. 2003; 27: 359-369.

4. Caspe SG, Moore DP, Leunda MR, Cano DB, Lischinsky L, Regidor-Cerrillo J, Álvarez-García G, Echaide IG, Bacigalupe D, Ortega Mora LM, Odeón AC, Campero CM. The Neospora caninum-Spain 7 isolate induces placental damage, fetal death and abortion in cattle when inoculated in early gestation. Vet Parasitol. 2012; 189(2-4): 171-81.

5. Cipolla AL, Casaro AP, Terzolo HR, Estela ES, Brooks BW, García MM. Persistence of Campylobacter fetus subspecies venerealis in experimentally infected heifers. Vet. Rec. 1994; 134: 628.

6. Di Rienzo JA, Casanoves F, Balzarini MG, Gonzalez L, Tablada M, Robledo YC. InfoStat versión 2020. [Software Estadístico] Grupo InfoStat, FCA, 2020. Universidad Nacional de Córdoba, Argentina. https://www.infostat.com.ar/index.php

7. Diamond LS. Lumen-dwelling protozoa: entamoeba, trichomonads and giarda. In vitro cultivation of Protozoan Parasites. In: Jensen J.B. (ed), CRC Press, Boca Raton, Florida. 1983; 89-98.

8. Felleisen RSJ. Comparative sequence analysis of 5.8S rRNA genes and internal transcribed spacer (ITS) regions of trichomonadid protozoa. Parasitology. 1997; 115: 111-119.

9. Fereig RM, Abdelbaky HH, Nishikawa Y. Comparative evaluation of four potent Neospora caninum diagnostic antigens using immunochromatographic assay for detection of specific antibody in cattle. Microorganisms. 2021; 9(10): 2133.

10. Garcia K, Weakley M, Do T, Mir S. Current and future molecular diagnostics of tick-borne diseases in cattle. Vet. Sci. 2022; 9(5): 241.

11. Givens MD, Marley MS. Infectious causes of embryonic and fetal mortality. Theriogenology. 2008; 70(3): 270-285.

12. Guarino A, Fusco G, Savini G, Di Francesco G, Cringoli G. Neosporosis in water buffalo (Bubalus bubalis) in southern Italy. Vet. Parasitol. 2000; 91: 15-21.

13. Guido S, Katzer F, Nanjiani I, Milne E, Innes EA. Serology based diagnostics for the control of bovine neosporosis. Trends Parasitol. 2016; 32: 131-143.

14. Hecker YP, Regidor Cerrillo J, Fiorani F, Horcajo P, Soria I, Gual I, Torioni S, Campero LM, Echaide IE, Álvarez García G, Ortega Mora LM, Zamorano PI, Venturini MC, Odeón AC, Cantón GJ, Moore DP. Immune response to Neospora caninum live tachyzoites in prepubertal female calves. Parasitol. Res. 2019; 118(10): 2945-2955.

15. Hecker YP, González Ortega S, Cano S, Ortega LM, Horcajo P. Bovine infectious abortion: a systematic review and meta-analysis. Front. Vet. Sci. 2023; 10: 1249410.

16. Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Bergey's Manual of Determinative Bacteriology. 9° ed. Microbiology. Williams & Wilkins. 1994, p. 602-606.

17. International Office of Epizootics [OIE]. Manual of diagnostic tests and vaccines for terrestrial animals (mammals, birds and bees). Vol 2. 6ª ed., OIE, Paris, 2008. p 599-1342.

18. Kalogianni AI, Stavropoulos I, Chaintoutis SC, Bossis I, Gelasakis AI. Serological, molecular and culture-based diagnosis of lentiviral infections in small ruminants. Viruses. 2021; 13(9): 1711.

19. Konrad JL, Campero LM, Caspe GS, Brihuega B, Draghi G, Moore DP, Crudeli GA, Venturini MC, Campero CM. Detection of antibodies against Brucella abortus, Leptospira spp., and Apicomplexa protozoa in water buffaloes in the Northeast of Argentina. Trop. Anim. Health Prod. 2013; 45: 1751-1756.

20. Moore DP, Campero CM, Odeón AC, Posso MA, Cano D, Leunda MR, Basso W, Venturini MC, Späth E, Seroepidemiology of beef and dairy herds and fetal study of Neospora caninum in Argentina. Vet. Parasitol. 2002. 107(4): 303-316.

21. Moore DP, Konrad JL, San Martino S, Reichel MP, Cano DB, Méndez S, Späth EJ, Odeón AC, Crudeli G, Campero CM. Neospora caninum serostatus is affected by age and species variables in cohabiting water buffaloes and beef cattle. Vet. Parasitol. 2014; 203(3-4): 259-263.

22. Mummah RO, Gomez AC, Guglielmino AH, Borremans B, Galloway RL, Prager KC, Lloyd Smith JO. Navigating cross-reactivity and host species effects in a serological assay: A case study of the microscopic agglutination test for Leptospira serology. PLoS Negl. Trop. Dis. 2024; 18(10): e0012042.

23. Nicola AM, Elena S, Franco C. Brucelosis (B. abortus, B. melitensis, B. suis) Manual de diagnóstico serológico. SENASA. 2019, 12-37.

24. Pulido Medellín MO, Garcia Corredor DJ, Vargas Abella JC. Seroprevalence of Neospora caninum in a dairy herd in boyacá, Colombia. Rev. Invest. Vet. Perú. 2016; 27(2): 355-362.

25. Reichel MP, Wahl LC, Hill FI. Review of Diagnostic Procedures and Approaches to Infectious Causes of Reproductive Failures of Cattle in Australia and New Zealand. Front. Vet. Sci. 2018; 5:222.

26. Seid U, Mohammed C, Ahmednur M, Weyzi J. Genetic disorders that cause stillbirth and abortion in cattle. Original Research Paper. 2021; 5(6): 3-8.

27. Truyers I, Luke T, Wilson D, Sargison N. Diagnosis and management of venereal campylobacteriosis in beef cattle. BMC Vet. Res. 2014; 10: 280.

28. Wheelhouse N, Howie F, Gidlow J, Greub G, Dagleish M, Longbottom D. Involvement of Parachlamydia in bovine abortions in Scotland. Vet. J. 2012; 193(2): 586-588.

29. Wilson Welder JH, Alt DP, Nally JE, Olsen SC. Bovine immune response to vaccination and infection with Leptospira borgpetersenii serovar Hardjo. Msphere. 2021; 6(2): 1110-1128.

30. Zhou J, Chen J, Peng Y, Xie Y, Xiao Y. A promising tool in serological diagnosis: Current research progress of antigenic epitopes in infectious diseases. Pathogens. 2022; 11(10): 1095.