Búsqueda de actividad biológica de aislados de fitoproteasas de Bromeliaceae nativas y cultivadas en Corrientes sobre microorganismos fitopatógenos

Melanie D. Gómez Herrera; Marina C. Cardozo; María C. Prieto; María V. Avanza; Paula Alayón Luaces

doi:10.30972/bon.3417883

Authors

Melanie D. Gómez Herrera Instituto de Química Básica y Aplicada del Nordeste Argentino- IQUIBA NEA (UNNE-CONICET), Facultad de Ciencias Exactas Naturales y Agrimensura, Universidad Nacional del Nordeste, Avenida Libertad 5470, Corrientes https://orcid.org/0000-0002-6401-7579
Marina C. Cardozo Instituto de Química Básica y Aplicada del Nordeste Argentino- IQUIBA NEA (UNNE-CONICET), Facultad de Ciencias Exactas Naturales y Agrimensura, Universidad Nacional del Nordeste, Avenida Libertad 5470, Corrientes https://orcid.org/0000-0002-6401-7579
María C. Prieto Departamento de Agroalimentos, Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba, Argentina, Félix A. Marrone 746, Ciudad Universitaria, Córdoba https://orcid.org/0000-0003-3314-3107
María V. Avanza Instituto de Química Básica y Aplicada del Nordeste Argentino- IQUIBA NEA (UNNE-CONICET), Facultad de Ciencias Exactas Naturales y Agrimensura, Universidad Nacional del Nordeste, Avenida Libertad 5470, Corrientes https://orcid.org/0000-0002-5680-9744
Paula Alayón Luaces Cátedra de Fruticultura, Departamento de Producción Vegetal, Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, Argentina, Sargento J. B. Cabral 2131, Corrientes https://orcid.org/0000-0002-5063-5923

DOI:

https://doi.org/10.30972/bon.3417883

Keywords:

Ananas comosus, Bromelia serra, natural control

Abstract

The aim of this work was to evaluate the biological activity of protein isolates from the native Bromelia serra (BS) and cultivated bromeliad Ananas comosus (AC) on phytopathogenic microorganisms. Samples of AC were obtained from the experimental station of the Universidad Nacional del Nordeste in Corrientes, Argentina and of BS from natural forests also in Corrientes, Argentina. Extracts of stem and leaves of AC and leaves of BS were processed through a precipitation with acetone, the pellet was re-suspended in autoclaved buffers and called protein isolates. The antimicrobial activity was evaluated by the disc diffusion technique, using phytopathogenic bacteria. However, an inhibition zone was not observed in any of the treatments with protein isolates, unlike the positive control with Streptomycin. The minimum inhibitory concentration of bacterial growth was determined by broth microdilution technique. The results showed that the protein isolates did not inhibit bacterial growth. Furthermore, antifungal activity against Fusarium oxysporum was evaluated by MTT method, but no significant inhibition was observed either. Although the protein extracts did not show biological activity against the microorganisms evaluated, future experiments will continue the evaluation by using other microorganisms. In addition, after a purification scheme will be established, different operational conditions and tests will be used with pure enzymes instead of their extracts.

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