Búsqueda de actividad biológica de aislados de fitoproteasas de Bromeliaceae nativas y cultivadas en Corrientes sobre microorganismos fitopatógenos

Melanie D. Gómez Herrera; Marina C. Cardozo; María C. Prieto; María V. Avanza; Paula Alayón Luaces

doi:10.30972/bon.3417883

Autores

Melanie D. Gómez Herrera Instituto de Química Básica y Aplicada del Nordeste Argentino- IQUIBA NEA (UNNE-CONICET), Facultad de Ciencias Exactas Naturales y Agrimensura, Universidad Nacional del Nordeste, Avenida Libertad 5470, Corrientes https://orcid.org/0000-0002-6401-7579
Marina C. Cardozo Instituto de Química Básica y Aplicada del Nordeste Argentino- IQUIBA NEA (UNNE-CONICET), Facultad de Ciencias Exactas Naturales y Agrimensura, Universidad Nacional del Nordeste, Avenida Libertad 5470, Corrientes https://orcid.org/0000-0002-6401-7579
María C. Prieto Departamento de Agroalimentos, Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba, Argentina, Félix A. Marrone 746, Ciudad Universitaria, Córdoba https://orcid.org/0000-0003-3314-3107
María V. Avanza Instituto de Química Básica y Aplicada del Nordeste Argentino- IQUIBA NEA (UNNE-CONICET), Facultad de Ciencias Exactas Naturales y Agrimensura, Universidad Nacional del Nordeste, Avenida Libertad 5470, Corrientes https://orcid.org/0000-0002-5680-9744
Paula Alayón Luaces Cátedra de Fruticultura, Departamento de Producción Vegetal, Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, Argentina, Sargento J. B. Cabral 2131, Corrientes https://orcid.org/0000-0002-5063-5923

DOI:

https://doi.org/10.30972/bon.3417883

Palavras-chave:

Ananas comosus, Bromelia serra, control natural

Resumo

El objetivo de este estudio es evaluar la actividad biológica de aislados proteicos de la planta nativa Bromelia serra (BS) y cultivada Ananas comosus (AC) sobre microorganismos fitopatógenos. Se obtuvieron muestras de bromeliáceas cultivadas (AC) del Campo Experimental de la Facultad de Ciencias Agrarias (UNNE) y de la bromeliácea nativa BS de montes naturales de la región. Se prepararon extractos de tallo y hojas de AC y hojas de BS, los cuales después de ser precipitados con acetona y obtener pellets, fueron resuspendidos en buffer estéril, los cuales se denominaron aislados proteicos. Se evaluó la actividad antimicrobiana frente a bacterias fitopatógenas mediante la técnica de difusión en disco. Sin embargo, no se observó la formación de un halo de inhibición en ninguno de los tratamientos con aislados proteicos, a diferencia del control positivo con estreptomicina. Se realizó la concentración inhibitoria mínima de crecimiento bacteriano por la técnica de microdilución en caldo. Los resultados mostraron

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