Efecto del resveratrol en el porcentaje y calidad de embriones in vitro generados por separación de blastómeras en bovinos

G. T. Portocarrero; J. V. Cortez; N. L. Murga; K. Beloti; R. Encina

doi:10.30972/vet.3215644

Authors

G. T. Portocarrero Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas. Laboratorio de Biotecnología Animal, Reproducción & Mejoramiento Genético.
J. V. Cortez Laboratorio Catalina Genetics.
N. L. Murga Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas. Laboratorio de Biotecnología Animal, Reproducción & Mejoramiento Genético.
K. Beloti Laboratorio Embryo Rio Preto.
R. Encina Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas. Laboratorio de Biotecnología Animal, Reproducción & Mejoramiento Genético.

DOI:

https://doi.org/10.30972/vet.3215644

Keywords:

clivage, embryonic development, total cells, living cells

Abstract

The oxidative state is an important factor that determines the development of bovine embryos. The objective in this study was to evaluate the effect of resveratrol on the quality of in vitro embryos generated by separation of blastomeres in cattle. Oocytes from the slaughterhouse were matured and fertilized in vitro by the conventional method. After 18 hours of fertilization, the zygotes were cultured for 3 days in synthetic oviductual fluid medium (SOF) for control and supplemented with 2 μM and 0.5 μM for the treatments. On day 3 they were stripped of their zona pellucida (ZP) to be cultivated at a rate of four blastomeres in well of the well (WOW) for 6 days in medium SOF supplemented with resveratrol. We evaluated percentage data of cleavage and embryonic division (8 to 10 blastomeres) after 3 days of culture, finding a significant difference p<0.05 with supplementation with 0.5 μM of resveratrol. At 6 days after blastomeres separation, percentage of embryos, number of total cells, live cells and dead cells were evaluated using Hoechst, FDA and PI staining respectively. There were no differences in the percentage of blastocysts between treatments; however, supplementation with 0.5 μM of resveratrol to the SOF medium had a greater amount of total cells and living cells (p<0.05). In conclusion, supplementation with resveratrol in the SOF medium does not increase the percentage of blastocysts but improves its quality using a concentration of 0.5 μM.

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