Effect of high-fat buffalo dairy diets with different fatty acid composition on hepatic fatty degeneration in C57BL/6J mice
DOI:
https://doi.org/10.30972/vet.3427040Keywords:
steatohepatitis, trans fats, ruminant, dairy fatAbstract
This work compared the effects of two diets high in milk fat of buffaloes with different fatty acid profiles on hepatic fatty degeneration in mice. Male C57BL/6J mice were fed for 154 days with balanced food (control) and two foods rich in milk fat (≈20%): one with a standard profile of fatty acids obtained from buffaloes fed with natural grass (GLP) and another reduced in saturated fats (-31%), rich in rumenic acid (+212,5%) and trans fats (+434,2%, predominantly vaccenic acid) (GLE), obtained from buffaloes fed with natural grass + lipid supplementation. Nutritional parameters, weight gain, cholesterolemia, serum transaminases, liver fat content, and liver histopathology were evaluated. Compared with the control group, GLP and GLE mice were obese, had increased serum ALT, and developed severe liver fatty degeneration (>66% affected parenchyma) with lobular inflammation and hepatocellular ballooning. There weren`t differences between the GLP and GLE groups in food and calorie intake, weight gain, markers of liver damage, cholesterolemia, hepatic lipid content or in liver lesions. The level of fat in the diet and not the type of milk fat was responsible for the observed changes. In conclusion, both high-fat buffalo dairy foods had a similar effect and neither attenuated liver fat degeneration of C57BL/6J mice.
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