Variabilidad de la interacción parásito-hospedador de diferentes genotipos Mus musculus al desafío experimental con Trichinella spiralis

S. González Beltrán; N. Orozco; G. Coscelli; P. Villanueva; M. I. Oyarzabal; C. Giudici

doi:10.30972/vet.3628324

Authors

S. González Beltrán Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Laboratorio de Trichinellosis. Centro de Investigación con Animales de Laboratorio (CIAL) https://orcid.org/0000-0002-8878-5580
N. Orozco Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Centro de Investigación con Animales de Laboratorio (CIAL) https://orcid.org/0009-0000-6162-5758
G. Coscelli Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Servicio de Diagnóstico Anatomopatológico https://orcid.org/0000-0001-8817-3536
P. Villanueva Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Centro de Investigación con Animales de Laboratorio (CIAL) https://orcid.org/0009-0002-6956-187X
M. I. Oyarzabal Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Centro de Investigación con Animales de Laboratorio (CIAL) https://orcid.org/0000-0001-5429-5789
C. Giudici Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias. Laboratorio de Trichinellosis https://orcid.org/0000-0002-1042-4638

DOI:

https://doi.org/10.30972/vet.3628324

Keywords:

Trichinella spiralis, mice, selection, weight, genetic variability, susceptibility

Abstract

Parasite-host interaction is a complex relationship that determines the outcome of infection. Trichinella spiralis is a nematode that affects a wide range of animals and can transmit infection to humans. Studying its interaction with the host is essential for developing strategies to reduce the impact of this parasitosis. The aim of this study was to evaluate potential differences in parasite-host interaction in response to a Trichinella spiralis challenge among different host genotypes, consisting of four mouse lines selected by body weight and a control line derived from the CF1 strain (s, h, s’, h’, and t, respectively). Male mice from the lines were infected with a dose of 400 muscle larvae. The expulsion index at 15 days post-infection indicated that the t line was the most efficient, whereas the h line was the least efficient in this process, showing the highest number of adult worms in the intestine. In the chronic stage of infection, the number of encysted muscle larvae (relative parasitic load) was significantly higher in the light h line and lower in the t and h’ lines, indicating differences in the host-parasite interaction among the genotypes. Histopathological evaluation of the duodenum also revealed differences among the studied lines. The observed variability may result from the systematic process of phenotypic artificial selection for body weight, along with dispersive effects - such as the founder effect and genetic drift - associated with the low effective population size.

Downloads

Download data is not yet available.

References

AVMA. Guidelines on Euthanasia of Animals: 2020 Edition. Disponible en: https://www.avma.org/sites/default/files/2020-02/Guidelines-on-Euthanasia-2020.pdf. Último acceso: enero 2025.

Bell RG. The generation and expression of immunity to Trichinella spiralis in laboratory rodents. Adv Parasitol. 1998; 41: 149-217.

Bell RG, Liu WM. Trichinella spiralis: quantitative relationship between intestinal worm burden, worm rejection, and measurement of intestinal immunity in inbred mice. Exp Parasitol. 1988; 66: 44-56.

Bernardi S, Brogliatti G, Oyarzabal MI. Diferencias de fertilidad en ratones seleccionados por peso. Analecta Veterinaria. 1999; 19(1/2): 11-7.

Bernardi S, Brogliatti G, Oyarzabal MI. Ovarian Structure in Mice Lines Selected for Weight. Anat Histol Embryol. 2009; 38: 200-3.

Bernardi S, Brogliatti G, Oyarzabal MI. Efectos de la Selección y de la Superovulación en las Pérdidas Gestacionales de Ratonas CF1. Int. J. Morphol. 2011; 29(1): 204-13.

Canadian Council on Animal Care. CCAC guidelines: Identification of scientific endpoints, humane intervention points, and cumulative endpoints 2022. Disponible en: https://ccac.ca/Documents/Standards/Guidelines/CCAC_guidelines_scientific_endpoints.pdf Último acceso: abril 2025

Dehlawi MS, Wakelin D. Parameters of intestinal inflammation in mice given graded infections of the nematode Trichinella spiralis. J Helminthol. 2002; 76(2):113-7.

Despommier DD. Trichinella spiralis and the concept of nich. J. Parasitol. 1993; 79(4): 472-82.

Fariña F, Pasqualetti MI, Bessi C, Ercole ME, Vargas C, Arbusti P, Ayesa G, Ribicich MM. Comparison between Trichinella patagoniensis and Trichinella spiralis infection in BALB/c mice. Vet Parasitol. 2020; 286.

Filbey KJ, Grainger JR, Smith KA, Boon L, van Rooijen N, Harcus Y, Jenkins E, Hewitson JP, Maizels RM. Innate and Adaptive Type 2 Immune Cell Responses in Genetically Controlled Resistance to Intestinal Helminth Infection. Immunol Cell Biol. 2014; 92: 436-48.

Garber J, Wayne R, Bielitzki J, Clayton LA, Donovan JC, Hendriksen C, Kohn D. Lipman N, Locke PA, Melcher J, Quimby FW, Turner PV, Wood GA, Würbel H. National Research Council (US). Committee for the Update of the Guide for the Care and Use of Laboratory Animals. Guide for the Care and Use of Laboratory Animals. 8th ed. Washington (DC): National Academies Press (US); 2011. PMID: 21595115.

Godoy JM, Codina AV, Kopp G, Vasconi MD, Di Masso RJ, Hinrichsen LI. Variación de los niveles séricos de interleuquinas (IL) durante la primoinfección con Trichinella spiralis (Ts) en ratones CBi-IGE susceptibles y resistentes al parásito. XVI Congreso y XXXIV Reunión Anual de la Sociedad de Biología de Rosario, Rosario, Argentina. 2014. p. 64.

Grencis RK. Immunity to Trichinella spiralis. PhD, University of Glasgow, Glasgow, Escocia. 1982. p. 14.

Harkness JE, Turner PV, Van de Woude S, Wheler CL. Harkness and Wagner’s Biology and Medicine of Rabbits and Rodents. 5th ed. Ames, Iowa, USA: Wiley‐Blackwell; 2010.

Kapel CMO, Gamble HR. Infectivity, persistence, and antibody response to domestic and sylvatic Trichinella spp. in experimentally infected pigs. Int J Parasitol. 2000; 30(2), 215-21.

Kobets T, Havelková H, Grekov I, Volkova V, Vojtíšková J, Slapničková M, Kurey I, Sohrabi Y, Svobodová M, Demant P, Lipoldová M. Genetics of Host Response to Leishmania tropica in Mice—Different Control of Skin Pathology, Chemokine Reaction, and Invasion into Spleen and Liver. PLoS Negl Trop Dis. 2012; 6, e1667.

Kutzer MAM, Armitage SAO. Maximising fitness in the face of parasites: a review of host tolerance. Zoology. 2016; 119: 281-89.

Lawrence CE, Paterson JCM, Higgins LM, MacDonald TT, Kennedy MW, Garside P. IL-4 regulated enteropathy in an intestinal nematode infection. Eur J Immunol. 1998; 28(9): 2672-84.

Luebke RW. Nematodes as host resistance models for detection of immunotoxicity. Methods. 2007; 41: 38-47.

Orozco N, González Beltrán S, Giudici C Oyarzabal MI. Resistencia y tolerancia a oxiuros en cuatro líneas de ratones seleccionados por peso. InVet. 2018; 20(1-2): 233-40.

Oyarzabal MI, Rabasa S. Selección divergente de peso en ratones con alta endocría seguida de 90 generaciones de cría libre. Mendeliana. 1995; 10(2): 119-32.

Oyarzabal MI, Rabasa S. Riqueza genética y estabilidad en ratones de la cepa CF1. Mendeliana. 1999; 13(2): 74-84.

Oyarzabal MI. Líneas de ratones originales como modelos experimentales en genética y mejoramiento animal. J Basic Appl Genet. 2011; 22, 1.

Pasqualetti M, Acerbo M, Miguez M, Rosa A, Fariña F, Cardillo N, Degregorio OJ, Ribicich M. Nuevos aportes al conocimiento de Trichinella y trichinellosis. Rev Med Vet. 2014; 95 (2):12-21.

Raberg L, Graham AL, Read AF. Decomposing health: tolerance and resistance to parasites in animals. Philos Trans R Soc Lond B Biol Sci. 2009; 364: 37-49.

Reiterová K, Antolová D, Hurníková Z. Humoral immune response of mice infected with low doses of Trichinella spiralis muscle larvae. Vet Parasitol. 2009; 159: 232-35.

Sánchez VG. Desarrollo de nuevos compuestos gemini con actividad antihelmíntica para aplicaciones veterinarias. PhD, Universidad Nacional del Litoral, Argentina. 2009.

Saracino MP, Vila CC, Cohen M, Gentilini MV, Falduto GH, Calcagno MA, Roux E, Venturiello SM, Malchiodi EL. Cellular and molecular changes and immune response in the intestinal mucosa during Trichinella spiralis early infection in rats. Parasit Vectors. 2020; 6, 13(1): 505.

Sokal RR, Rohlf J. Biometría: principios y métodos estadísticos en la investigación biológica. H. Madrid: Blume; 1979. p. 832.

Vasconi MD, Malfante P, Bassi A, Giudici C, Revelli S, Di Masso R, Font MT, Hinrichsen L. Phenotypic differences on the outcome of the host-parasite relationship: behaviour of mice of the CBi stock in natural and experimental infections. Vet Parasitol. 2008;153: 157-63.

Vasconi MD, Bertorini G, Codina AV, Indelman P, Di Masso RJ, Hinrichsen LI. Phenotypic Characterization of the Response to Infection with Trichinella spiralis in Genetically Defined Mouse Lines of the CBi-IGE Stock. Open J of Vet Med. 2015; 5: 111-22.

Wakelin D. Genetic control of immunity to parasites. Infection with Trichinella spiralis in inbred and congenic mice showing rapid and slow responses to infection. Parasite Immunol. 1980; 2: 85-98.

Watanabe N. The effect of inheritance of IgE responsiveness on the susceptibility of mice to Trichinella spiralis infection. Front. Immunol. 2023; 14:1185094.

Yu YR, Deng MJ, Lu WW, Jia MZ, Wu w, Qi YF. Systemic cytokine profiles and splenic toll-like receptor expression during Trichinella spiralis infection. Exp Parasitol. 2013; 134: 92-101.