Un nuevo método para la conservación de animales evita el uso de formaldehído y mejora las características de conservación

C. F. Pereyra; D. G. Parola; M. E. Biancardi; L. C. Pérez Mogetta; M. N. Cirimele; V. L. Venegas

doi:10.30972/vet.3628512

Authors

C. F. Pereyra Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias https://orcid.org/0000-0001-9207-7264
D. G. Parola Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias https://orcid.org/0009-0001-7509-6102
M. E. Biancardi Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas https://orcid.org/0009-0008-2894-2848
L. C. Pérez Mogetta Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias https://orcid.org/0009-0000-1541-0318
M. N. Cirimele Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias https://orcid.org/0009-0005-2138-8248
V. L. Venegas Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias https://orcid.org/0009-0006-5944-7669

DOI:

https://doi.org/10.30972/vet.3628512

Keywords:

Tissue preservation, eugenol, anatomical techniques, CFP-Soft Fix, veterinary anatomy

Abstract

Dr. W. Thiel developed a human cadaver preservation technique that maintains essential features
such as tissue flexibility, elasticity, volume, and color, using minimal amounts of formaldehyde. This method
enables clear anatomical recognition without significant alterations in the preserved material. The aim of
this study was to evaluate the application of the CFP-Soft Fix specimen preservation method—a modified,
formaldehyde-free version of Thiel’s technique—to the locomotor system of New Zealand rabbits. Twenty
rabbits were used. Pelvic members were extracted and preserved. The effectiveness of the modified technique
was assessed by examining parameters such as joint flexibility, muscle elasticity, muscle volume, tissue
color, and presence of fungal or bacterial contamination. Additionally, a histological analysis of muscle and
tendon tissues was performed. The solution proved to be non-irritating and nearly odorless, while effectively
preserving color, flexibility, and plasticity, closely resembling live tissue. The preserved specimens exhibited
excellent joint flexibility, good tissue elasticity, nearly normal muscle volume, and well-preserved histological
muscle structure. The CFP–Soft Fix method successfully eliminated formaldehyde from the preservation
process and demonstrated adaptability for use in domestic animals. This technique allows for safe anatomical
study, supports the use of complementary tools such as arthroscopy, laparoscopy, endoscopy, and diagnostic
imaging, and reinforces the importance of detailed anatomical knowledge in clinical education by improving
recognition of topographic structures and spatial relationships.

Downloads

Download data is not yet available.

References

American Veterinary Medical Association. AVMA Guidelines for the euthanasia of animals. Schaumburg, Illinois, EEUU. Leary S, Underwood W, Anthony R, Cartner S, Corey C, Grandin T, et al., Editores. 2013.

Benkhadra M, Bouchot A, Gérard J, Genelot D, Trouilloud P, Martin L, Girard C, Danino A, Anderhuber F, Feigl G. Flexibility of Thiel’s embalmed cadavers: the explanation is probably in the muscles. Surg Radiol Anat. 2011; 33(4): 365-8.

Berke JH. Cytologic examination of the nasal mucosa in formaldehyde-exposed workers. J Occup Med. 1987; 29(8): 681-4.

Bertone V, Blasi E, Ottone N, Dominguez M. Walther Thiel Method for the Preservation of Corpses with Maintenance of the main physical properties of Vivo. Rev. argent. anat. online. 2011; 2: 71-100.

Clark G. Staining Procedures. 4th ed. Baltimore: Biological Stain Commission (U.S.) Williams & Wilkins; 1981.

De Segura I. Métodos de anestesia, analgesia y eutanasia. Madrid: La Paz; 2007.

Eisma R, Lamb C, Soames RW. From formalin to thiel embalming: What changes? One anatomy department’s experiences. Clin Anat. 2013; 26(5): 564-71.

García del Moral R. Laboratorio de anatomía patológica. Madrid, España: McGraw-Hill Interamericana; 1993.

Hammer N, Löffler S, Bechmann I, Steinke H, Hädrich C, Feja C. Comparison of modified Thiel embalming and ethanol-glycerin fixation in an anatomy environment: Potentials and limitations of two complementary techniques. Anat Sci Educ. 2015; 8(1): 74-85.

Hayashi S, Homma H, Naito M, Oda J, Nishiyama T, Kawamoto A, Kawata S, Sato N, Fukuhara T, Taguchi H, Mashiko K. Saturated salt solution method: a useful cadaver embalming for surgical skills training. Medicine. 2014; 93(27): e196.

Hayashi S, Naito M, Kawata S, Qu N, Hatayama N, Hirai S, Itoh M. History and future of human cadaver preservation for surgical training: from formalin to saturated salt solution method. Anat Sci Int. 2016; 91(1): 1-7.

Healy SE, Rai BP, Biyani CS, Eisma R, Soames RW, Nabi G. Thiel embalming method for cadaver preservation: a review of new training model for urologic skills training. Urology. 2015; 85(3): 499-504.

Kerckaert I, Hoof T, Pattyn P, D’Herde K. Endogent: Centre for anatomy and invasive techniques. anatomy (International journal of Experimental and Clinical Anatomy). 2008; 1.

König HE, Probst A, Dier H, Sora C. Production of anatomical specimens for teaching practice in veterinary anatomy by means of polyethylene glycole (PEG) impregnation. A comparison with the method of plastination. Agrociencia. 2013; 29: 23-8.

Molina Aragonés JM, Bausà Peris R, Carreras Valls R, Carrillo Castillo A, Fiblà Nicolau F, Gaynés Palou E, Guerrero Monge J, Inglés Torruella J, López Muñoz JA, Martinez Martínez-Carrasco E, Matllo Aguilar J. Toxicidad del formaldehido en trabajadores profesionalmente expuestos. Revisión bibliográfica. Arch Prev Riesgos Labor. 2018; 21(3): 128-57.

Parodi TV, Cunha MA, Heldwein CG, de Souza DM, Martins ÁC, Garcia LD, Junior WW, Monserrat JM, Schmidt D, Caron BO, Heinzmann. The anesthetic efficacy of eugenol and the essential oils of Lippia alba and Aloysia triphylla in post-larvae and sub-adults of Litopenaeus vannamei (Crustacea, Penaeidae). Comp Biochem Physiol C Toxicol Pharmacol. 2012; 155(3): 462-8.

Pulido Guerrero JA, Valderrama Sandoval JA. Determinación de la concentración mínima inhibitoria de Formaldehído capaz de disminuir el crecimiento bacteriano de cepas obtenidas en piscinas de conservación cadavérica. Bogotá: Javeriana Facultad De Ciencias Carrera de Microbiología. 2007; 20 SRC: 48-9.

Sugata Y, Miyaso H, Odaka Y, Komiyama M, Sakamoto N, Mori C, Matsuno Y. Levels of formaldehyde vapor released from embalmed cadavers in each dissection stage. E nviron. Sci. Pollut. Res. 2016; 23(16): 16176-82.

Thiel W. Die Konservierung ganzer Leichen in natürlichen Farben. Ann Anat. 1992; 174: 185-95.

Weber WA, Weiglein A, Latorre R, Henry R. Polyester plastination of Biological tissue: P35 technique. J Int Soc Plastination. 2007; 22: 50-8.

Zúñiga J, Marí Tur J, Milocco S. Ciencia y tecnología en protección y experimentación animal. 1st ed. Madrid, España: Mc Graw-Hill Interamericana; 2001.