Evaluación de dos concentraciones de glicerol y dimetilformamida en la criopreservación de semen de llama (Lama glama)

F. Salas Champi; E. Ampuero; C. Ordóñez; A. Meza; H. Cucho

doi:10.30972/vet.3628608

Authors

F. Salas Champi Universidad Nacional de San Antonio Abad del Cusco. Facultad de Agronomía y Zootecnia. Escuela Profesional de Zootecnia. Laboratorio de Biotecnologías Reproductivas del Centro Experimental La Raya
E. Ampuero Universidad Nacional de San Antonio Abad del Cusco. Facultad de Agronomía y Zootecnia. Escuela Profesional de Zootecnia. Laboratorio de Biotecnologías Reproductivas del Centro Experimental La Raya https://orcid.org/0000-0001-6159-7451
C. Ordóñez Universidad Nacional de San Antonio Abad del Cusco. Facultad de Agronomía y Zootecnia. Escuela Profesional de Zootecnia. Laboratorio de Biotecnologías Reproductivas del Centro Experimental La Raya https://orcid.org/0000-0002-2955-4555
A. Meza Universidad Nacional de San Antonio Abad del Cusco. Facultad de Agronomía y Zootecnia. Escuela Profesional de Zootecnia. Laboratorio de Biotecnologías Reproductivas del Centro Experimental La Raya https://orcid.org/0000-0001-8581-9987
H. Cucho Universidad Nacional de San Antonio Abad del Cusco. Facultad de Agronomía y Zootecnia. Escuela Profesional de Zootecnia. Laboratorio de Biotecnologías Reproductivas del Centro Experimental La Raya https://orcid.org/0000-0001-7170-9795

DOI:

https://doi.org/10.30972/vet.3628608

Keywords:

Llama, semen, cryopreservation, glycerol, dimethylformamide

Abstract

The objective of this study was to evaluate the effect of two concentrations of glycerol (5% and 7%) and dimethylformamide (DMF; 7% and 9%) on the successful cryopreservation of llama semen collected by electroejaculation (EE). Sixteen ejaculates were obtained from eight adult males (4-7 years old). Semen samples were incubated with papain for 20 min to reduce viscosity, and the enzymatic action was subsequently inhibited before fresh evaluation. Samples were diluted in a Tris-based medium and gradually cooled to 5 °C over 2.5 h. They were then divided into four aliquots for cryoprotectant addition, evaluated during the cooling phase, packaged in straws, frozen within 25 min, and stored for 7 days prior to thawing. Sperm analysis was performed using a CASA-Mot system (ISAS®), assessing total motility (TM), kinematic parameters, sperm viability, and acrosomal integrity in fresh (papain-incubated), cooled, and thawed samples. Data was analyzed using a randomized complete block design, with mean comparisons performed by Tukey’s test (p<0.05). Results showed that TM, viability, and acrosomal integrity were significantly higher (p<0.05) in fresh semen compared to samples treated with glycerol or DMF during the cooling and post-thaw stages. Moreover, curvilinear velocity and amplitude of lateral head displacement were higher in fresh semen and in samples treated with 5% and 7% glycerol during cooling. In conclusion, none of the evaluated concentrations of glycerol or DMF effectively preserved sperm parameters after cryopreservation.

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