Reduction of in vitro viral replication of caprine alphaherpesvirus 1 (CPHV-1) through natural antiviral treatment with plant extracts

C. Ferreccio; S. Maidana; R. Tau; L. Di Ciaccio; V. Spotorno; A. Salvat; J. J. Aguilar; B. Konigheim; S. A. Romera

doi:10.30972/vet.3618088

Autores

C. Ferreccio Institute of Virology and Technological Innovations (IVIT) https://orcid.org/0009-0003-2137-2102
S. Maidana Institute of Virology and Technological Innovations (IVIT). Universidad del Salvador. Faculty of Agricultural and Veterinary Sciences. Chair of Applied Immunology https://orcid.org/0000-0001-7449-9359
R. Tau Institute of Virology and Technological Innovations (IVIT) https://orcid.org/0009-0005-2574-7324
L. Di Ciaccio Institute of Pathobiology, INTA (Instituto Nacional de Tecnología Agropecuaria) https://orcid.org/0009-0003-7742-4223
V. Spotorno Institute of Biological Resources, INTA (Instituto Nacional de Tecnología Agropecuaria) https://orcid.org/0009-0000-5361-3941
A. Salvat Institute of Pathobiology, INTA (Instituto Nacional de Tecnología Agropecuaria) https://orcid.org/0000-0002-4700-2527
J. J. Aguilar Institute of Biological Resources, INTA (Instituto Nacional de Tecnología Agropecuaria) https://orcid.org/0000-0002-9605-0189
B. Konigheim Institute of Biological Resources, INTA (Instituto Nacional de Tecnología Agropecuaria) https://orcid.org/0000-0003-1724-2156
S. A. Romera Institute of Virology and Technological Innovations (IVIT). Universidad del Salvador. Faculty of Agricultural and Veterinary Sciences. Chair of Applied Immunology https://orcid.org/0000-0002-3966-9399

DOI:

https://doi.org/10.30972/vet.3618088

Palavras-chave:

herpesvius, treatment, L. divaricata, M. verticillata, P. quadrangularis

Resumo

Caprine herpesvirus 1 (CpHV-1) is distributed worldwide, causing significant economic losses. The application of acyclovir interferes with viral replication to varying degrees of efficacy, but it does not prevent the establishment of latency. Due to the costs and side effects associated with synthetic drugs, the search for new chemotherapeutic agents is essential for the treatment and control of viral diseases. The objective of this study is to evaluate and compare the in vitro antiviral activity of three native plants from Argentina as a therapeutic antiviral treatment against CpHV-1. Larrea divaricata, Minthostachys verticillata and Parastrephia quadrangularis were used. Four parameters were analyzed: Cytotoxic Concentration 50 (CC50), Inhibitory Concentration 50 (IC50), quantification by plaque assay and viral titration. The L. divaricata extract showed the lowest cytotoxicity (2.4 mg ml-1). The best selectivity index (SI) was obtained with the M. verticillata extract (10). L. divaricata and P. quadrangularis extracts demonstrated the greatest antiviral effects, with reductions of 90% and 75%, respectively, compared to untreated controls, where the greatest reduction in plaque size was observed. The highest inhibition of viral replication observed in the viral titration quantification occurred when monolayers were treated with L. divaricata, resulting in at least a 35% reduction. The combination of assay with L. divaricata and M. verticillata showed the greatest reduction in viral titers. The overall results provide evidence that L. divaricata and M. verticillata could serve as potential sources for new anti-CpHV-1 drugs.

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Referências

.

Alonso MR, Marrassini C, Saint Martin M, Cogoi L, Peralta I, Anesini C. Larrea divaricata a native Argentine plant with potential activity against SARS-CoV-2? Bol Latinoam Caribe Plant Med Aromat. 2023; 22(6): 747-769.

Álvarez SG, Dubed EM, Noa RE, Navea LLM, Pérez Guevara MT, Rodríguez A. Validación del método de titulación del virus de la inmunodeficiencia humana tipo 1. Rev Cubana Med Trop. 2009; 61(2): 1-9.

Anesini C, Boccio J, Cremaschi G, Genaro A, Zubillaga M, Sterin Borda L, Borda ES. In vivo antitumoural activity and acute toxicity study of Larrea divaricata Cav. extract. Phytother Res. 1997; 11: 521-523.

Ardiles A, Barrientos R, Simirgiotis MJ, Bórquez J, Sepúlveda B, Areche C. Gastroprotective activity of Parastrephia quadrangularis (Meyen), cabrera from the atacama desert. Molecules. 2018; 23: 2361.

Ayma L, Hervé D, Sauvain M. Efecto del extracto acuoso de la Ch’illka (Parastrephia lucida) en el control de la sarna en llamas. In: Genin D, Picht HJ, Lizarazu R, Rodriguez T. (Eds.). Orstom. Conpac-Oruro. IBTA, 1995, pp. 185-194.

Basbaum AI, Bautista DM, Scherrer G, Julius D. Cellular and molecular mechanisms of pain. Cell. 2009; 139(2): 267-84.

Bigliani MC, Grondona E, Zunino PM, Ponce AA. Effects of Cecropia pachystachya and Larrea divaricata aqueous extracts in mice. Hum Exp Toxicol. 2010; 29: 601-606.

Borenfreund E, Puerner JA. Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol Lett. 1985; 24(2-3): 119-124.

Camero M, Bellacicco AL, Tarsitano E, Decaro N, Martella V, Tempesta M, Buonavoglia C. Intravaginal administration of an inactivated vaccine prevents lesions induced by caprine herpesvirus-1 in goats. Vaccine. 2007; 25(9): 1658-61.

Camero M1, Crescenzo G, Marinaro M, Tarsitano E, Bellacicco AL, Armenise C, Buonavoglia C, Tempesta M. Cidofovir does not prevent caprine herpesvirus type-1 neural latency in goats. Antivir Ther. 2010; 15(5): 785-8.

Camero M, Buonavoglia D, Lucente MS, Losurdo M, Crescenzo G, Trerotoli P, Casalino E, Martella V, Elia G, Tempesta M. Enhancement of the antiviral activity against caprine herpesvirus type 1 of Acyclovir in association with Mizoribine. Res Vet Sci. 2017; 111: 120-123.

Camero M, Lanave G, Catella C, Capozza P, Gentile A, Fracchiolla G, Britti D, Martella V, Buonavoglia C, Tempesta M. Virucidal activity of ginger essential oil against caprine alphaherpesvirus-1. Vet Microbiol. 2019; 230: 150-155.

Candanosa IE, AIE, Sierra GM, Sánchez CA, SGG, Méndez BA, Cobos ML, Álvarez Ramírez L. Vulvovaginitis pustular sugerente a herpesvirus caprino- 1 en cabras (Querétaro, México). Vet. Méx. 2011; 42(3): 233-243.

Cariddi LN, Panero A, Demo MS, Sabini LI, Maldonado AM. Inhibition of immediate-type allergic reaction by Minthostachys verticillata (griseb.) epling essential oil. J. Essent Oil Res. 2007; 19(2): 190-196.

Chen H, Teng L, Li JN, Park R, Mold DE, Gnabre J, Hwu JR, Tseng WN, Huang RC. Antiviral activities of methylated nordihydroguaiaretic acids. 2. Targeting herpes simplex virus replication by the mutation insensitive transcription inhibitor Tetra-O-methyl-NDGA. J. Med Chem. 1998; 41(16): 3001-3007.

D’Almeida RE, Alberto MR, Quispe C, Schmeda-Hirschmann G, Isla MI. Antimicrobial phenylpropanoids from the Argentinean highland plant Parastrephia lucida (Meyen) Cabrera. J Ethnopharmacol. 2012; 142(2): 407-414.

Davison AJ. Herpesvirus systematics. Vet Microbiol. 2010; 143(1): 52-69.

Di Ciaccio LS, Spotorno VG, Córdoba Estévez MM, Ríos DJL, Fortunato RH, Salvat AE. Antifungal activity of Parastrephia quadrangularis (Meyen) Cabrera extracts against Fusarium verticillioides. Lett Appl Microbiol. 2018; 66(3): 244-251.

Echiburu-Chau C, Pastén L, Parra C, Bórquez J, Mocan A, Simirgiotis MJ. High resolution UHPLC-MS characterization and isolation of main compounds from the antioxidant medicinal plant Parastrephia lucida (Meyen). Saudi Pharm J. 2017; 25(7): 1032-1039.

Engels M, Gelderblom H, Darai G, Ludwig H. Goat herpesviruses: Biological and physicochemical properties. J Gen Virol. 1983; 64(10): 2237-2247.

Engels M, Loepfe E. Wild P, Schraner E, Wyler R. The genome of caprine herpesvirus 1: Genome structure and relatedness to bovine herpesvirus 1. J Gen Virol.1987; 68(7): 2019-2023.

Escobar FM, Magnoli A, Sabini MC, Cariddi LN, Bagnis G, Soltermann A, Cavaglier L. Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity. J. Appl. Anim. Res.2019; 47(1): 217-222.

Grewal AS, Wells R. Vulvovaginitis of goats due to a herpesvirus. Aust Vet J. 1986; 63(3): 79-82.

He T, Tang Q, Zeng N, Gou L, Liu JW, Yang J, Yu L, Wang Z, Gong XP. Study on effect and mechanism of volatile oil of schizonepetae herba and its essential components against influenza virus. Zhongguo Zhong Yao Za Zhi. 2013; 38(11): 1772-7.

Helwig NE, Hong S, Hsiao-wecksler ET. Estudio de la actividad antibacteriana de la fracción butanólica de Passiflora quadrangularis en aislamientos clínicos de Helicobacter pylori de origen colombiano. Rep Inst Javeriano. 2018; 1-7.

Hilfiger L, Triaux Z, Marcic C, Héberlé E, Emhemmed F, Darbon P, Marchioni E, Petitjean H, Charlet A. Anti-Hyperalgesic Properties of Menthol and Pulegone. Front Pharmacol. 2021; 12: 753873.

Keuser V, Gogev S, Schynts F, Thiry E. Demonstration of generalized infection with caprine herpesvirus 1 diagnosed in an aborted caprine fetus by PCR. Vet Res Commun. 2002; 26(3): 221-226.

Konigheim B, Aguilar J, Goleniowski M, Contigiani M. Antiviral activity of Larrea divaricata extracts on Junin virus. Mol Med Chem. 2006; 10: 12-13.

Ladelfa F, Del Médico Zajac MP, Kotsias F, Delgado F, Muylkens TJ, Thiry E, Romera SA. Comparative study on the in vitro and in vivo properties of two bovine herpesvirus-5 reference strains. Acta Vet Scand. 2011; 53: 37.

Lanave G, Zizzadoro C, Trerotoli P, Martella V, Buonavoglia C , Tempesta M , Camero M Antiviral activity of PHA767491 on Caprine alphaherpesvirus 1 in vitro. Res Vety Sci. 2019; 126: 113-117.

Lü JM, Nurko J, Weakley SM, Jiang J, Kougias P, Lin PH, Yao Q, Chen C. Molecular mechanisms and clinical applications of nordihydroguaiaretic acid (NDGA) and its derivatives: An update. Med Sci Monit. 2010; 16(5): RA93-R100.

Martinez F, Mugas ML, Aguilar JJ, Marioni J, Contigiani MS, Núñez Montoya SC, Konigheim BS. First report of antiviral activity of nordihydroguaiaretic acid against Fort Sherman virus (Orthobunyavirus). Antiviral Res. 2021; 187: 104976.

Martinez F, Ghietto LM, Lingua G, Mugas ML, Aguilar JJ, Gil P, Pisano MB, Marioni J, Paglini MG, Contigiani MS, Núñez-Montoya SC, Konigheim BS. New insights into the antiviral activity of nordihydroguaiaretic acid: Inhibition of dengue virus serotype 1 replication. Phytomedicine. 2022; 106: 154424.

Montagnaro S, Ciarcia R, De Martinis C, Pacilio C, Sasso S, Puzio MV, De Angelis M, Pagnini U, Boffo S, Kenez I, Iovane G, Giordano A. Modulation of apoptosis by caprine herpesvirus 1 infection in a neuronal cell line. J Cell Biochem. 2013; 114(12): 2809-2822.

Ordaz-Trinidad N, Dorantes-Álvarez L, Salas-Benito, J, Barrón-Romero BL, Salas-Benito M, Nova-Ocampo MD. Citotoxicidad y actividad antiviral de extractos de chiles (Capsicum spp). Polibot. 2018; (46): 273-285.

Palavecino Ruiz MDP, Ordóñeza RM, Isla MI, Sayago JE. Activity and mode of action of Parastrephia lepidophylla ethanolic extracts on phytopathogenic fungus strains of lemon fruit from Argentine Northwest. Postharvest Biol and Technol. 2016; 114: 62-68.

Park R, Giza PE, Mold DE, Huang RCC. Inhibition of HSV-1 replication and reactivation by the mutation-insensitive transcription inhibitor tetra-O-glycyl-nordihydroguaiaretic acid. Antiviral Res. 2003; 58(1): 35-45.

Pasquereau S, Nehme Z, Haidar Ahmad S, Daouad F, Van Assche J, Wallet C, Schwartz C, Rohr O, Morot-Bizot S, Herbein G. Resveratrol inhibits hcov-229e and sars-cov-2 coronavirus replication in vitro. Viruses. 2021; 13(2): 354.

Peralta I, Martino R, Davicino R, Gorzalczany S, Alonso R, Anesini C. Systemic and local toxicity assay of an aqueous extract of Larrea divaricata Cav.: Role of NDGA. Int. J. Pharm. Sci. Res. 2015; 6(7): 2790-2798.

Peralta I, Marrassini C, Saint Martin M, Cogoi L, Alonso MR, Gugliucci A, Anesini C. Larrea divaricata: anti-inflammatory and antioxidant effects of on macrophages and low-density lipoproteins. BMC Comp Med and Therapies. 2022; 22(1): 84.

Primo V, Rovera M, Zanon S, Oliva M, Demo M, Daghero J, Sabini L. Determination of the antibacterial and antiviral activity of the essential oil from Minthostachys verticillata (Griseb.) Epling. Rev Argent Microbiol. 2001; 33(2): 113-117.

Quintans JSS, Shanmugam S, Heimfarth L, Araújo AAS, Almeida JRGDS, Picot L, Quintans-Júnior LJ. Monoterpenes Modulating Cytokines - A Review. Food Chem. Toxicol. 2019; 123: 233-257.

Reed L, Muench H. Asimple method of estimating fifty per cent endopoints l ’ 2. In: The American Journal of Hygiene. 1938; 27(3). Disponible en: https://academic.oup.com/aje/article-abstract/27/3/493/99616

Rodríguez Basso A, Carranza A, Zainutti VM, Bach H, Gorzalczany SB. Pharmacologycal activity of peperina (Minthostachys verticillata) on gastrointestinal tract. J Ethnopharmacol. 2021; 269: 113712.

Saz Peiró P, Saz Tejero S. Revisión de tratamientos naturales en infecciones por el HERPES. Med Nat 2012; 6(1): 30-38.

Schuhmacher A, Reichling J, Schnitzler P. Virucidal effect of peppermint oil on the enveloped viruses herpes simplex virus type 1 and type 2 in vitro. Phytomed. 2003; 10(6-7): 504-510.

Sim LY Abd Rani NZ, Husain K. Lamiaceae: An insight on their anti-allergic potential and its mechanisms of action. Front in Pharmacol. 2019; 10: 667.

Soria N, Ramos P. Uso de plantas medicinales en la Atención Primaria de Salud en Paraguay: algunas consideraciones para su uso seguro y eficaz. Mem. Inst. Invest. Cienc. Salud (Impr.). 2015; 13(2): 8-17.

Soto-Acosta R, Bautista-Carbajal P, Syed GH, Siddiqui A, Del Angel RM. Nordihydroguaiaretic acid (NDGA) inhibits replication and viral morphogenesis of dengue virus. Antiviral Res. 2014; 109(1): 132-140.

Stege PW, Davicino RC, Vega AE, Casali YA, Correa S, Micalizzi B. Antimicrobial activity of aqueous extracts of Larrea divaricata Cav (jarilla) against Helicobacter pylori. Phytomed. 2006; 13(9-10): 724-727.

Tarigan S, Webb RF, Kirkland D. Caprine herpesvirus from balanoposthitis. Aust Vet J. 1987; 64(10): 321.

Tempesta M, Pratelli A, Greco G, Martella V, Buonavoglia C. Detection of caprine herpesvirus 1 in sacral ganglia of latently infected goats by PCR. J Clin Microbiol. 1999; 37(5): 1598-1599.

Tempesta M, Pratelli A, Normanno G, Camero M, Buonavoglia D, Greco G, Buonavoglia C. Experimental intravaginal infection of goats with caprine herpesvirus 1. J Vet Med B. 2000; 47(3): 197-201.

Tempesta M, Camero M, Greco G, Pratelli A, Martella V, Buonavoglia C. A classical inactivated vaccine induces protection against caprine herpesvirus 1 infection in goats. Vaccine. 2001; 19(28-29): 3860-3864.

Tempesta M1, Camero M, Bellacicco AL, Tarsitano E, Crescenzo G, Thiry J, Martella V, Decaro N, Elia G, Neyts J, Thiry E, Buonavoglia C. Potent inhibition of genital herpesvirus infection in goats by cidofovir. Antivir Ther. 2007; 12(6): 977-9.

Tempesta M, Crescenzo G, Camero M, Bellacicco AL, Tarsitano E, Decaro N, Neyts J, Martella V, Buonavoglia C. Assessing the efficacy of cidofovir against herpesvirus-induced genital lesions in goats using different therapeutic regimens. Antimicrob Agents Chemother. 2008; 52(11): 4064-4068.

Visintini Jaime MF, Redko F, Muschietti LV, Campos RH, Martino VS, Cavallaro LV. In vitro antiviral activity of plant extracts from Asteraceae medicinal plants. Virol J. 2013; 10: 245.

Vivanco, RFC, and Edwin RAB. Natural antioxidant anti-influenza composition. U.S. Patent No. 9,770,477. 2017.

Vogt MV, Sutil SB, Escobar FM, Sabini MC, Cariddi LN, Torre CV, Zanon SM, Sabini LI. Minthostachys verticillata essentials oil and its major components: antiherpetic selective action in HEp-2 cells. Molecular Med. Chem. 2010; 21: 117-120.

Whitley RJ, Roizman B. Herpes simplex virus infections. Lancet. 2001; 357(9267): 1513-1518.

Zampini IC, Sayago J. Antioxidant and xanthine oxidase inhibitory activities of plant species from the Argentina Puna (Antofagasta, Catamarca). Pharmacol. Ther. 2008; 21: 90-103.

Zampini IC, Cuello S, Alberto MR, Ordoñez RM, D’ Almeida R, Solorzano E, Isla MI. Antimicrobial activity of selected plant species from “the Argentine Puna” against sensitive and multi-resistant bacteria. J Ethnopharmacol. 2009; 124(3): 499-505.