Prevalence of microorganisms and antimicrobial resistance profile in dairy cattle of Paraguay
DOI:
https://doi.org/10.30972/vet.3215629Keywords:
bovine, mastitis, microbial isolation, antimicrobial resistanceAbstract
The bovine mastitis is an inflammation of the mammary glands of endemic presentation mainly caused by the invasion of pathogenic microorganisms. The objective of the study was to identify the most frequently isolated microorganisms and to determine the antimicrobial resistance profile according to the species or group of species of microorganisms isolated. The 411 milk samples were taken from equal numbers of California Mastitis Test CMT-positive quarter cows from dairy breeds in 3 departments of Paraguay (224, 137 and 50 from the Central, Cordillera and Paraguarí departments), which were subjected to microbial species identification cultures. Microbial sensitivity to antimicrobials was evaluated by plate dilution. The absolute and relative frequency of microorganisms and resistances were quantified with the Epiinfo 7.0 software. The 37.4% of the microorganisms corresponded to Streptocccus sp, 35.3% to the coagulase negative Staphylococcus group (SCN) and 16.1% to the coagulase positive Staphylococcus (SCP) as the most frequent. The antimicrobial resistance profile in decreasing order was tetracycline, penicillin, sulfatrimoprim, rifampicin and ampicillin (34.7; 19.7; 14.4; 8.2 and 6.4%, respectively). Streptocccus sp were resistant to 7/10 (range 51.3 to 1.7%), SCN to 8/10 (range 29.7 to 2%), and SCP to 7/10 (range 44.7 to 2.1%), while enterobacteria were resistant to 7/20, mainly to ampicillin (4/5). Multiresistance was also observed, although in a small number. It was concluded that the most frequent groups of microorganisms were Streptocccus sp, followed by SCN and thirdly SCP. Antimicrobial resistance, from highest to lowest, was to tetracycline, penicillin and sulfatrimetoprim, being still rare multiresistance.
Downloads
References
Barkema HW, Schukken YH, Zadoks RN. 2006. Invited Review: the role of cow, pathogen, and treatment regimen in the therapeutic success of bovine Staphylococcus aureus mastitis, J Dairy Sci 89: 1877-1895.
Blowey RW, Edmondson PW. 1995. Mastitis control in dairy herds. Farming Press, Ipswich, p. 29.
Boireau C et al. 2018. Antimicrobial resistance in bacteria isolated from mastitis in dairy cattle in France, 2006-2016. J Dairy Sci 101: 9451-9462.
Bonetto CC. 2014. Mastitis bovina causada por Staphylococcus
coagulasa negativos. Tesis de Doctorado, Univ. Nac. de la Plata, Argentina, 206 p.
Clinical & Laboratory Standards Institute. 2013. Performance
standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals Approved standard and supplement VET01-A4 and VET01-S2 (replaces M31 A3) (Fourth Edition). Wayne, PA: Clinical Laboratory Standard Institute.
Contreras GA, Rodríguez J M. 2011. Mastitis: comparative etiology and epidemiology. J Mammary Gland Biol Neoplasia 16: 339-356.
Cheng J et al. 2019. Antimicrobial resistance profiles of 5 common bovine mastitis pathogens in large Chinese dairy herds. J Dairy Sci 102: 2416-2426.
Edmondson P. 2011. Blitz therapy for the eradication of Streptococcus agalactiae infections in dairy cattle. In Practice 33: 33-37.
Elgarch F et al. 2020. Antimicrobial susceptibility of nine udder pathogens recovered from bovine clinical mastitis milk in Europe 2016. Vet Microbiol 245: 1-13.
Florentín CC. 2007. Perfil de resistencia in vitro a antimicrobianos
de cepas causantes de mastitis aisladas de leche cruda bovina en establecimientos de pequeña y mediana producción. Mem Inst Investig Cienc Salud 5: 19-25.
Gao J et al. 2012 Molecular types and antibiotic resistance of Staphylococcus aureus isolates from bovine mastitis in a single herd in China, Vet J 192: 550-552.
Gizaw F et al. 2020. Distribution and antimicrobial resistance
profile of coagulase-negative staphylococci from cattle, equipment, and personnel on dairy farm and abattoir settings. Heliyon 6 e03606: 1-11.
Halasa T et al. 2007. Economic effects of bovine mastitis and mastitis management: A review. Vet Q 29: 18-31.
Jiménez VS et al. 2020. Perfil de resistencia antimicrobiana
en aislamientos de Staphylococcus sp. obtenidos de leche bovina en Colombia. Rev Argent Microbiol 52: 121-130.
Koneman EW, Allen S, Janda WM, Schreckenberger PC, Winn WC. 1999. Diagnóstico Microbiológico, 5º Ed., Panamericana, Madrid, p. 528-529.
Kroning IS et al. 2016. Staphylococcus aureus isolated from handmade sweets: biofilm formation, enterotoxigenicity and antimicrobial resistance. Food Microbiol 58: 105-111.
McDougall S, Hussein H, Petrovski K. 2014. Antimicrobial resistance in Staphylococcus aureus, Streptococcus uberis and Streptococcus dysgalactiae from dairy cows with mastitis. N. Z. Vet. J. 62: 68-76.
National Mastitis Council. 2018. Global milk quality. procedures for collecting milk samples. On line: http://www.nmconline.org/sampling.htm
OMS. 2016. Plan de acción mundial sobre la resistencia a los antimicrobianos. Acceso. 25-8-2020. On line: https://www.who.int/antimicrobial-resistance/publica tions/global-
action-plan/es/
Raspanti CG et al. 2016. Prevalence and antibiotic susceptibility
of coagulase-negative Staphylococcus species from bovine subclinical mastitis in dairy herds in the central region of Argentina. Rev Argent Microbiol 48: 50-56.
Rato MG et al. 2013. Antimicrobial resistance and molecular
epidemiology of streptococci from bovine mastitis. Vet. Microbiol 161: 286-294.
Sánchez MP, Gutiérrez NP. 2015. Frecuencia y susceptibilidad
antimicrobiana del estafilococo coagulasa negativo aislado de mastitis bovina en fincas lecheras del Tolima, Colombia. Rev Med Vet Bogota: 30: 83-93.
Sawant AA, Gillespie BE, Oliver SP. 2009. Antimicrobial susceptibility of coagulase negative Staphylococcus species isolated from bovine milk, Vet Microbiol 134: 73–81.
Schroeder JW. 2012. Bovine mastitis and milking management,
NDSU Extention Serv 1129: 1-16.
Srednik ME, Abate S, Gentilini ER. 2016. Susceptibilidad antibiótica de estafilococos aislados de muestras de leche provenientes de mastitis bovina. In Vet 18: 39-44.
Swedberg G, Ringertz S, Skold O. 1998. Sulfonamide resistance in Streptococcus pyogenes is associated with differences in the amino acid sequence of its chromosomal dihydropteroate synthase. Antimicrob Agents Chemother 42: 1062-1067.
Taponen S, Nykäsenoja S, Pohjanvirta T, Pitkälä A, Pyörälä S. 2016. Species distribution and in vitro antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine mastitic milk. Acta Vet Scand 58: 1-13.
Vanderhaeghen W et al. 2015. Identification, typing, ecology and epidemiology of coagulase negative staphylococci associated with ruminants. Vet J 203: 44-51.
Zhang S et al. 2018. Phenotypic and genotypic characterization
of antimicrobial resistance profiles in Streptococcus dysgalactiae isolated from bovine clinical mastitis in 5 provinces of China. J Dairy Sci 101: 3344-3355.
Downloads
Published
How to Cite
Issue
Section
License
Revista Veterinaria (Rev. Vet.) maintains a commitment to the policies of Open Access to scientific information, as it considers that both scientific publications as well as research investigations funded by public resources should circulate freely without restrictions. Revista Veterinaria (Rev. Vet.) ratifies the Open Access model in which scientific publications are made freely available at no cost online.
.jpg){kind=logo}
.jpg){kind=logo}
