Prevalence of microorganisms and antimicrobial resistance profile in dairy cattle of Paraguay
DOI:
https://doi.org/10.30972/vet.3215629Keywords:
bovine, mastitis, microbial isolation, antimicrobial resistanceAbstract
The bovine mastitis is an inflammation of the mammary glands of endemic presentation mainly caused by the invasion of pathogenic microorganisms. The objective of the study was to identify the most frequently isolated microorganisms and to determine the antimicrobial resistance profile according to the species or group of species of microorganisms isolated. The 411 milk samples were taken from equal numbers of California Mastitis Test CMT-positive quarter cows from dairy breeds in 3 departments of Paraguay (224, 137 and 50 from the Central, Cordillera and Paraguarí departments), which were subjected to microbial species identification cultures. Microbial sensitivity to antimicrobials was evaluated by plate dilution. The absolute and relative frequency of microorganisms and resistances were quantified with the Epiinfo 7.0 software. The 37.4% of the microorganisms corresponded to Streptocccus sp, 35.3% to the coagulase negative Staphylococcus group (SCN) and 16.1% to the coagulase positive Staphylococcus (SCP) as the most frequent. The antimicrobial resistance profile in decreasing order was tetracycline, penicillin, sulfatrimoprim, rifampicin and ampicillin (34.7; 19.7; 14.4; 8.2 and 6.4%, respectively). Streptocccus sp were resistant to 7/10 (range 51.3 to 1.7%), SCN to 8/10 (range 29.7 to 2%), and SCP to 7/10 (range 44.7 to 2.1%), while enterobacteria were resistant to 7/20, mainly to ampicillin (4/5). Multiresistance was also observed, although in a small number. It was concluded that the most frequent groups of microorganisms were Streptocccus sp, followed by SCN and thirdly SCP. Antimicrobial resistance, from highest to lowest, was to tetracycline, penicillin and sulfatrimetoprim, being still rare multiresistance.
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