Aplicación de benzoato de estradiol como alternativa a la gonadotrofina coriónica equina en protocolos de sincronización de celos en ovinos

M. V. Gómez; A. T. Soto; R. L. de la Sota

doi:10.30972/vet.3628740

Authors

M. V. Gómez Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de investigaciones en Reproducción Animal https://orcid.org/0000-0002-7737-0873
A. T. Soto Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de investigaciones en Reproducción Animal https://orcid.org/0000-0003-2300-4728
R. L. de la Sota Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de investigaciones en Reproducción Animal https://orcid.org/0000-0002-9417-9560

DOI:

https://doi.org/10.30972/vet.3628740

Keywords:

Estradiol benzoate dosage, GnRH, artificial insemination, sheep

Abstract

In sheep, traditional estrus synchronization (ES) protocols involve the insertion of a progesterone- or progestogen-containing device, followed by the administration of equine chorionic gonadotropin (eCG) at device removal to induce ovulation. Previous research evaluating estradiol benzoate (EB) as an alternative to eCG has reported low pregnancy rates. The objectives of this study were to determine (a) the optimal EB dose (100 vs. 200 μg) and timing of administration (0, 24, or 36 h after device removal) within an ES protocol (Experiments I and II), and (b) the pregnancy rate obtained using ES protocols with progesterone plus either eCG or EB, combined with fixed-time artificial insemination (FTAI; Experiment III), in ewes during the breeding season. Experiments I and II indicated that the optimal EB treatment was 100 μg administered at 0 h. In Experiment III, pregnancy rates did not differ between eCG- and EB-based ES protocols. These findings suggest that EB can replace eCG in ES protocols with FTAI at 50–55 h using fresh semen, although further research is needed to refine the timing of FTAI.

Downloads

Download data is not yet available.

References

AL-Jaryan IL, AL-Thuwaini TM, Merzah LH, Alkhammas AH. Reproductive physiology and advanced technologies in sheep reproduction. Rev. Agric. Sci. 2023; 11: 171-180.

Ali A, Derar DR. Relationships among the corpus luteum, follicles and conceptus in sheep. Anat. Histol. Embryol. 2021; 50(1): 65-71.

Brogni CF, de Souza AF. Ultrasonography in sheep follicular dynamics. OAJAR. 2021; 1(01): 25-29.

Castilho C, Ferreira de Almeida M, Giometti IC, Zoccolaro Costa M, Almeida Gabriel Filho LR, Gardim De Cesare A. Use of estradiol benzoate to induce ovulation in a short-term protocol for fixed-time AI in sheep. Semina: Cienc. Agrar. 2015; 36(3): 1419-1423.

Driancourt MA, Gibson WR, Cahill LP. Follicular dynamics throughout the oestrous cycle in sheep. A review. Reprod. Nutr. Dev. 1985; 25(1A): 1-15.

Edmondson MA, Shipley CF. Theriogenology of Sheep, Goats, and Cervids. In: Pugh DG, Baird AN, Edmondson M, Passler T, editors. Sheep, Goat, and Cervid MEDICINE. 3rd ed. Canada: Elsiever; c2022. p.1441-207.

Feng T, Ding H, Wang J, Xu W, Liu Y, Kenéz Á. Alterations of serum metabolites and fecal microbiota involved in ewe follicular cyst. Front. Microbiol. 2021; 12: 675480.

Gurung NK, Rush J, Pugh DG. Feeding and Nutrition. In: Pugh DG, Baird AN, Edmondson M, Passler T, editors. Sheep, Goat, and Cervid MEDICINE. 3rd ed. Canada: Elsiever; 2022. p.15-43.

Hameed N, Khan MIR, Zubair M, Andrabi SMH. Approaches of estrous synchronization in sheep: developments during the last two decades: a review. Trop. Anim. Health Prod. 2021; 53(485): 1-10.

Hazlinda H, Salleh SM, Panandam JM, Nasir SM, Yaakub H. Effect of fluorogestone acetate (FGA) sponge with oestradiol benzoate (OB) on oestrus response and behaviour in Dorper cross ewes. Mal. J. Anim. Sci. 2021; 24(1): 76-82.

Madrigali A, Rota A, Panzani D, Castellani S, Shawahina M, Hassan A, Camillo F. Artificial insemination in sheep with fresh diluted Semen: and management protocols. Trop. Anim. Sci. J. 2021: 44(3): 255-260.

Manteca Vilanova X, De Briyne N, Beaver B, Turner PV. Horse welfare during equine chorionic gonadotropin (eCG) production. Animals. 2019; 9(12): 1053.

Mapletoft RJ, Baruselli PS, Bo GA. Fisiología relacionada al uso de la eCG en ganado de carne y leche. SPERMOVA. 2022; 12(1): 83-91.

Martemucci G, D’Alessandro AG. Estrous and fertility responses of dairy ewes synchronized with combined short term GnRH, PGF2α and estradiol benzoate treatments. Small Rum. Res. 2010; 93(1): 41-47.

Murphy BD. Equine chorionic gonadotropin: an enigmatic but essential tool. AR. 2018; 9(3): 223-230.

Olivera-Muzante J, Fierro S, López V, Gil J. Comparison of prostaglandin and progesterone-based protocols for time artificial insemination in sheep. Theriogenology. 2011; 75(7): 1232-1238.

SAS Institute. 2020. Base SAS 9.4 procedures guide. SAS Institute.

Shaw L, Phillips K. Guide to providing optimal nutrition to ewes throughout the year. 2023. In Practice. 2023; 45(10): 610-618.

Silveira DC, Vargas SF, Oliveira FC, Barbosa RM, Knabah NW, Goularte KL, Vieira AD, Baldassarre H, Gasperin BG, Mondadori RG, Lucia T. Pharmacological approaches to induce follicular growth and ovulation for fixed-time artificial insemination treatment regimens in ewes. Anim. Reprod. Scie. 2021; 228: 106734.